URL: http://www.addgene.org/10957
Proper Citation: RRID:Addgene_10957
Insert Name: MDR1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:2898143
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pHa; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA, has the wild-type glycine at position 185 on P-glycoprotein (see GenBank ID M14758.1 for the sequence variations at nucleotides 382, 964, 978, 979, 1660 and 2065 that are present in this plasmid) The "Ha" in pHaMDRwt stand for the Harvey sarcoma virus. Vector can confer the complete multidrug-resistance phenotype to various drug sensitive cell lines. Figure legend for author's map: Structure of retroviral vector carrying MDR] cDNA. sites: B, BamHI; E, EcoRI; H, HindIII; S, Stu I; X, Xho I. bp, Base pairs; amp, ampicillin resistance gene.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org