URL: http://www.addgene.org/105829
Proper Citation: RRID:Addgene_105829
Insert Name: Humanized EAziRS
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:28771403
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The plasmid has been developed for efficient incorporation of Azi into GPCRs, first used in Seidel et al. eLIFE 2017. With respect to similar plasmids from other labs, this plasmid contains a humanized gene for EAziRS and 4 copies of the U6-BstYam tRNA cassette right downstream of the synthetase cassette.
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Source: Addgene