URL: http://www.addgene.org/105782
Proper Citation: RRID:Addgene_105782
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29590189
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp-In competent; Bacterial Resistance:Ampicillin
Comments: This vector is a member of the pKK vector family. Its cloning site was optimized for the SLIC approach, however, a traditional restriction and ligation based approach is also possible (see Szczesny RJ et al. for detailed protocols; bioRxiv, doi: https://doi.org/10.1101/160101). This vector is a derivate of the pcDNA5/FRT/TO plasmid (Invitrogen) and is suitable for stable cell line generation using the Flp-In system. Transcription of the gene of interest is driven by a tetracycline responsive CMV promoter. A given coding sequence can be cloned into all pKK vectors using just three universal PCR primers and a ligation-independent DNA cloning method. CyOFP1 has large Stokes shift. It is excited in the blue and emits in the orange range. It can be multiplexed with EGFP in fluorescence based assays. Also as acceptor for blue-emitting luciferase (PMID: 27240196). CyOFP1 coding sequence was cloned from Addgene ID 74279.
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Source: Addgene