URL: http://www.addgene.org/105215
Proper Citation: RRID:Addgene_105215
Insert Name: Vpr
Organism: HIV-1
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29046351
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The pMM310-BlamVpr plasmid reagent was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: pMM310(Cat#11444) from Dr. Michael Miller. The DNA sequences encoding for Beta-lactamase (Blam) was replaced with HindIII-mCherry-PC-NotI-PC-YFP-BamHI encoding sequences. mCherry-pc2x-eYFPVpr plasmid reference: Sood C, Francis AC, Desai TM, Melikyan GB.An improved labeling strategy enables automated detection of single-virus fusion and assessment of HIV-1 protease activity in single virions.J Biol Chem. 2017 Oct 18. pii: jbc.M117.818088. doi: 10.1074/jbc.M117.818088. pMM310 plasmid reference: Tobiume M, Lineberger JE, Lundquist CA, Miller MD, and Aiken C. Nef Does Not Affect the Efficiency of Human Immunodeficiency Virus Type 1 Fusion with Target Cells. J. Virology 77(19); 10645-50, 2003. [Abstract]. An in-depth description of a similar system: Cavrois M, De Noronha C, Greene WC. A sensitive and specific enzyme-based assay detecting HIV-1 virion fusion in primary T lymphocytes. Nat Biotechnol. 2002 Nov;20(11):1151-4. Epub 2002 Sep 30. [Abstract]
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org