URL: http://www.addgene.org/105215
Proper Citation: RRID:Addgene_105215
Insert Name: Vpr
Organism: HIV-1
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29046351
Vector Backbone Description: Backbone Size:5015; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The pMM310-BlamVpr plasmid reagent was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: pMM310(Cat#11444) from Dr. Michael Miller. The DNA sequences encoding for Beta-lactamase (Blam) was replaced with HindIII-mCherry-PC-NotI-PC-YFP-BamHI encoding sequences. mCherry-pc2x-eYFPVpr plasmid reference: Sood C, Francis AC, Desai TM, Melikyan GB.An improved labeling strategy enables automated detection of single-virus fusion and assessment of HIV-1 protease activity in single virions.J Biol Chem. 2017 Oct 18. pii: jbc.M117.818088. doi: 10.1074/jbc.M117.818088. pMM310 plasmid reference: Tobiume M, Lineberger JE, Lundquist CA, Miller MD, and Aiken C. Nef Does Not Affect the Efficiency of Human Immunodeficiency Virus Type 1 Fusion with Target Cells. J. Virology 77(19); 10645-50, 2003. [Abstract]. An in-depth description of a similar system: Cavrois M, De Noronha C, Greene WC. A sensitive and specific enzyme-based assay detecting HIV-1 virion fusion in primary T lymphocytes. Nat Biotechnol. 2002 Nov;20(11):1151-4. Epub 2002 Sep 30. [Abstract]
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Source: Addgene