URL: http://www.addgene.org/104585
Proper Citation: RRID:Addgene_104585
Insert Name: UCP1 promoter
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28930673
Vector Backbone Description: Backbone Marker:Origene; Vector Backbone:pCMV6-AC-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The CMV promoter driving GFP expression was removed and replaced with the -5.5 kb mouse UCP1 promoter + Kozak Reference sequence for promoter may be found here: http://epd.vital-it.ch/cgi-bin/get_doc?db=mmEpdNew&format=genome&entry=Ucp1_1
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org