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Anti-PTB (1) Monoclonal Antibody, Unconjugated, Clone 1

RRID:AB_86977

Antibody ID

AB_86977

Target Antigen

PTB (1) human, mouse, rat

Proper Citation

(Innovative Research Cat# 32-4800, RRID:AB_86977)

Clonality

monoclonal antibody

Reference

PMID:27565344

Comments

manufacturer recommendations: Immunofluorescence; Immunoprecipitation; Western Blot; WB, IF, IP

Clone ID

Clone 1

Host Organism

mouse

Vendor

Innovative Research

Cat Num

32-4800

Publications that use this research resource

Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex.

  • Zhang X
  • Cell
  • 2016 Aug 25

Literature context:


Abstract:

Alternative splicing is prevalent in the mammalian brain. To interrogate the functional role of alternative splicing in neural development, we analyzed purified neural progenitor cells (NPCs) and neurons from developing cerebral cortices, revealing hundreds of differentially spliced exons that preferentially alter key protein domains-especially in cytoskeletal proteins-and can harbor disease-causing mutations. We show that Ptbp1 and Rbfox proteins antagonistically govern the NPC-to-neuron transition by regulating neuron-specific exons. Whereas Ptbp1 maintains apical progenitors partly through suppressing a poison exon of Flna in NPCs, Rbfox proteins promote neuronal differentiation by switching Ninein from a centrosomal splice form in NPCs to a non-centrosomal isoform in neurons. We further uncover an intronic human mutation within a PTBP1-binding site that disrupts normal skipping of the FLNA poison exon in NPCs and causes a brain-specific malformation. Our study indicates that dynamic control of alternative splicing governs cell fate in cerebral cortical development.