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Goat Anti-Human FRAP (N-19) Polyclonal, Unconjugated antibody

RRID:AB_631981

Antibody ID

AB_631981

Target Antigen

Human FRAP1 human

Proper Citation

(Santa Cruz Biotechnology Cat# sc-1549, RRID:AB_631981)

Clonality

polyclonal antibody

Comments

Discontinued: 2016; validation status unknown check with seller; recommendations: ELISA; Immunofluorescence; Immunoprecipitation; Western Blot; Western Blotting, Immunoprecipitation, Immunofluorescence, ELISA

Clone ID

N-19

Host Organism

goat

Vendor

Santa Cruz Biotechnology

Cat Num

sc-1549

Publications that use this research resource

LARP1 functions as a molecular switch for mTORC1-mediated translation of an essential class of mRNAs.

  • Hong S
  • Elife
  • 2017 Jun 26

Literature context:


Abstract:

The RNA binding protein, LARP1, has been proposed to function downstream of mTORC1 to regulate the translation of 5'TOP mRNAs such as those encoding ribosome proteins (RP). However, the roles of LARP1 in the translation of 5'TOP mRNAs are controversial and its regulatory roles in mTORC1-mediated translation remain unclear. Here we show that LARP1 is a direct substrate of mTORC1 and Akt/S6K1. Deep sequencing of LARP1-bound mRNAs reveal that non-phosphorylated LARP1 interacts with both 5' and 3'UTRs of RP mRNAs and inhibits their translation. Importantly, phosphorylation of LARP1 by mTORC1 and Akt/S6K1 dissociates it from 5'UTRs and relieves its inhibitory activity on RP mRNA translation. Concomitantly, phosphorylated LARP1 scaffolds mTORC1 on the 3'UTRs of translationally-competent RP mRNAs to facilitate mTORC1-dependent induction of translation initiation. Thus, in response to cellular mTOR activity, LARP1 serves as a phosphorylation-sensitive molecular switch for turning off or on RP mRNA translation and subsequent ribosome biogenesis.

Funding information:
  • NIDDK NIH HHS - R01 DK083491()
  • NIGMS NIH HHS - R01 GM088565()
  • NIGMS NIH HHS - R01 GM110019()