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Boc Polyclonal Antibody

RRID:AB_2716570

Antibody ID

AB_2716570

Target Antigen

Boc mouse

Proper Citation

(Frédéric Charron Lab; Institut de Recherches Cliniques de Montreal; Canada Cat# SM272, RRID:AB_2716570)

Clonality

polyclonal antibody

Comments

PMID:20053908 "We also generated a rabbit anti-Boc antibody directed against the cytoplasmic tail of mouse Boc."

Host Organism

rabbit

Vendor

Frédéric Charron Lab; Institut de Recherches Cliniques de Montreal; Canada

Cat Num

SM272

Publications that use this research resource

Sonic Hedgehog Is a Remotely Produced Cue that Controls Axon Guidance Trans-axonally at a Midline Choice Point.

  • Peng J
  • Neuron
  • 2018 Jan 17

Literature context:


Abstract:

At the optic chiasm choice point, ipsilateral retinal ganglion cells (RGCs) are repelled away from the midline by guidance cues, including Ephrin-B2 and Sonic Hedgehog (Shh). Although guidance cues are normally produced by cells residing at the choice point, the mRNA for Shh is not found at the optic chiasm. Here we show that Shh protein is instead produced by contralateral RGCs at the retina, transported anterogradely along the axon, and accumulates at the optic chiasm to repel ipsilateral RGCs. In vitro, contralateral RGC axons, which secrete Shh, repel ipsilateral RGCs in a Boc- and Smo-dependent manner. Finally, knockdown of Shh in the contralateral retina causes a decrease in the proportion of ipsilateral RGCs in a non-cell-autonomous manner. These findings reveal a role for axon-axon interactions in ipsilateral RGC guidance, and they establish that remotely produced cues can act at axon guidance midline choice points.