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Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546

RRID:AB_2535745

Antibody ID

AB_2535745

Target Antigen

Human IgG (H+L) Cross-Adsorbed human

Proper Citation

(Thermo Fisher Scientific Cat# A-21089, RRID:AB_2535745)

Clonality

polyclonal antibody

Comments

Applications: IHC (1-10 µg/mL), ICC (1-10 µg/mL), IF (1-10 µg/mL)

Host Organism

goat

Vendor

Thermo Fisher Scientific Go To Vendor

Cat Num

A-21089

Publications that use this research resource

Immune or Genetic-Mediated Disruption of CASPR2 Causes Pain Hypersensitivity Due to Enhanced Primary Afferent Excitability.

  • Dawes JM
  • Neuron
  • 2018 Feb 21

Literature context:


Abstract:

Human autoantibodies to contactin-associated protein-like 2 (CASPR2) are often associated with neuropathic pain, and CASPR2 mutations have been linked to autism spectrum disorders, in which sensory dysfunction is increasingly recognized. Human CASPR2 autoantibodies, when injected into mice, were peripherally restricted and resulted in mechanical pain-related hypersensitivity in the absence of neural injury. We therefore investigated the mechanism by which CASPR2 modulates nociceptive function. Mice lacking CASPR2 (Cntnap2-/-) demonstrated enhanced pain-related hypersensitivity to noxious mechanical stimuli, heat, and algogens. Both primary afferent excitability and subsequent nociceptive transmission within the dorsal horn were increased in Cntnap2-/- mice. Either immune or genetic-mediated ablation of CASPR2 enhanced the excitability of DRG neurons in a cell-autonomous fashion through regulation of Kv1 channel expression at the soma membrane. This is the first example of passive transfer of an autoimmune peripheral neuropathic pain disorder and demonstrates that CASPR2 has a key role in regulating cell-intrinsic dorsal root ganglion (DRG) neuron excitability.

Funding information:
  • NINDS NIH HHS - NS18400(United States)