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Anti-Kir4.1 (extracellular) antibody

RRID:AB_2341043

Antibody ID

AB_2341043

Target Antigen

Kir4.1 (extracellular) See NCBI gene human, mouse, rat

Proper Citation

(Alomone Labs Cat# APC-165, RRID:AB_2341043)

Clonality

polyclonal antibody

Reference

PMID:29606582

Comments

Useful for Immunocytochemistry, Western blot, Live cell imaging, Indirect flow cytometry, Immunohistochemistry, Immunoprecipitation

Host Organism

rabbit

Vendor

Alomone Labs

Cat Num

APC-165

Publications that use this research resource

Kir4.1-Dependent Astrocyte-Fast Motor Neuron Interactions Are Required for Peak Strength.

  • Kelley KW
  • Neuron
  • 2018 Apr 18

Literature context:


Abstract:

Diversified neurons are essential for sensorimotor function, but whether astrocytes become specialized to optimize circuit performance remains unclear. Large fast α-motor neurons (FαMNs) of spinal cord innervate fast-twitch muscles that generate peak strength. We report that ventral horn astrocytes express the inward-rectifying K+ channel Kir4.1 (a.k.a. Kcnj10) around MNs in a VGLUT1-dependent manner. Loss of astrocyte-encoded Kir4.1 selectively altered FαMN size and function and led to reduced peak strength. Overexpression of Kir4.1 in astrocytes was sufficient to increase MN size through activation of the PI3K/mTOR/pS6 pathway. Kir4.1 was downregulated cell autonomously in astrocytes derived from amyotrophic lateral sclerosis (ALS) patients with SOD1 mutation. However, astrocyte Kir4.1 was dispensable for FαMN survival even in the mutant SOD1 background. These findings show that astrocyte Kir4.1 is essential for maintenance of peak strength and suggest that Kir4.1 downregulation might uncouple symptoms of muscle weakness from MN cell death in diseases like ALS.

Funding information:
  • FIC NIH HHS - K01 TW000001(United States)