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SIF antibody

RRID:AB_2315325

Antibody ID

AB_2315325

Target Antigen

Proper Citation

(A. Yasuda, Suntory Institute for Bioorganic Research; Osaka; Japan Cat# SIF, RRID:AB_2315325)

Clonality

unknown

Reference

PMID:21826660

Vendor

A. Yasuda, Suntory Institute for Bioorganic Research; Osaka; Japan

Cat Num

SIF

Publications that use this research resource

Toward a single-cell-based analysis of neuropeptide expression in Periplaneta americana antennal lobe neurons.

  • Neupert S
  • J. Comp. Neurol.
  • 2012 Mar 1

Literature context:


Abstract:

A multitude of potential neurotransmitters and neuromodulators, including peptides, have been detected in the antennal lobe (AL), the first synaptic relay of the central olfactory pathway in the insect brain. However, the functional role of neuropeptides in this system has yet to be revealed. An important prerequisite to understanding the role of neuropeptides is to match the functionally different cell types in the AL with their peptide profiles by using electrophysiological recordings combined with immunocytochemical studies and/or single-cell mass spectrometry. The olfactory system of Periplaneta americana is particularly well suited to accomplish this goal because several physiologically distinct neuron types can be unequivocally identified. With the aim to analyze the neuropeptide inventory of the P. americana AL, this study is an essential step in this direction. First, we systematically analyzed different parts of the AL by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry to obtain the complete set of neuropeptides present. Altogether, 56 ion signals could be assigned to products of 10 neuropeptide genes (allatostatins A, B, C, SIFamide, allatotropin, FMRFamide-related peptides [myosuppressin, short neuropeptides F, extended FMRFamides], crustacean cardioactive peptide, tachykinin-related peptides). In a second step, a combination of immunocytochemistry and mass spectrometric profiling of defined AL compartments was used to reveal the spatial distribution of neuropeptide-containing cells. Finally, we demonstrated the feasibility of MALDI-TOF mass spectrometric profiling of single AL neurons, which is an important precondition for combining electrophysiology with peptide profiling at the single-cell level.

Funding information:
  • Intramural NIH HHS - Z99 CA999999(United States)
  • NIGMS NIH HHS - DP2 GM119139(United States)