X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Anti-phospho-MYPT1 (Thr696) antibody

RRID:AB_11212365

Antibody ID

AB_11212365

Target Antigen

phospho-MYPT1 (Thr696) h, m, pm, r, ca, xn, eq, ch, horse, chicken/bird, xenopus/amphibian, non-human primate

Proper Citation

(Millipore Cat# ABS45, RRID:AB_11212365)

Clonality

polyclonal antibody

Reference

PMID:29706577

Comments

seller recommendations: Western Blot; WB

Host Organism

rabbit

Vendor

Millipore

Cat Num

ABS45

Publications that use this research resource

Spred1 Safeguards Hematopoietic Homeostasis against Diet-Induced Systemic Stress.

  • Tadokoro Y
  • Cell Stem Cell
  • 2018 May 3

Literature context:


Abstract:

Stem cell self-renewal is critical for tissue homeostasis, and its dysregulation can lead to organ failure or tumorigenesis. While obesity can induce varied abnormalities in bone marrow components, it is unclear how diet might affect hematopoietic stem cell (HSC) self-renewal. Here, we show that Spred1, a negative regulator of RAS-MAPK signaling, safeguards HSC homeostasis in animals fed a high-fat diet (HFD). Under steady-state conditions, Spred1 negatively regulates HSC self-renewal and fitness, in part through Rho kinase activity. Spred1 deficiency mitigates HSC failure induced by infection mimetics and prolongs HSC lifespan, but it does not initiate leukemogenesis due to compensatory upregulation of Spred2. In contrast, HFD induces ERK hyperactivation and aberrant self-renewal in Spred1-deficient HSCs, resulting in functional HSC failure, severe anemia, and myeloproliferative neoplasm-like disease. HFD-induced hematopoietic abnormalities are mediated partly through alterations to the gut microbiota. Together, these findings reveal that diet-induced stress disrupts fine-tuning of Spred1-mediated signals to govern HSC homeostasis.

Funding information:
  • Arthritis Research UK - 17522(United Kingdom)