URL: http://antibodyregistry.org/AB_11134475
Proper Citation: (Icosagen AS Cat# 302-100, RRID:AB_11134475)
Target Antigen: Mouse monoclonal antibody to human CDNF
Clonality: monoclonal
Comments: manufacturer recommendations: IgG1 ELISA, WB ELISA: 1:64 000; WB: 1:4000 Monoclonal antibody workingtiter has to be established practically for each particular antigen and assayformat
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No rating or validation information has been found for Mouse monoclonal antibody to human CDNF.
Possibly Non-Specific
Extracted Antibody Information: "all used at 1:500 dilution. The localization pattern of infused rhCDNF in the striatum and cortex was confirmed with another anti-CDNF antibody derived from mouse (catalog #302-100, Icosagen AS; RRID: AB_11134475)."
Extracted Specificity Statement: "E–H, Chromogenic detection of anti-CDNF IR was used to analyze the SNc on sections from uninfused (E), rhCDNF-infused (F), or 6-OHDA-treated rhCDNF-infused hemispheres (G; n = 5–6). Sections from CDNF-deficient mice were used to reveal unspecific staining by the anti-CDNF antibody (H). Arrowheads mark the nuclei of non-neuronal cells nonspecifically binding the anti-CDNF antibody."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:28275710
Possibly Non-Specific
Extracted Antibody Information: "all used at 1:500 dilution. The localization pattern of infused rhCDNF in the striatum and cortex was confirmed with another anti-CDNF antibody derived from mouse (catalog #302-100, Icosagen AS; RRID: AB_11134475)."
Extracted Specificity Statement: "Sections from CDNF-deficient mice were used to reveal unspecific staining by the anti-CDNF antibody (H). Arrowheads mark the nuclei of non-neuronal cells nonspecifically binding the anti-CDNF antibody. Arrows mark some of the cells that contain punctate anti-CDNF IR."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:28275710
Possibly Non-Specific
Extracted Antibody Information: "all used at 1:500 dilution. The localization pattern of infused rhCDNF in the striatum and cortex was confirmed with another anti-CDNF antibody derived from mouse (catalog #302-100, Icosagen AS; RRID: AB_11134475)."
Extracted Specificity Statement: "After chromogenic detection of anti-CDNF IR, all samples displayed cross-reactivity of the anti-CDNF antibody with an epitope in the nuclei of non-neuronal cells. The unspecific nature of this anti-CDNF IR in the nuclei of non-neuronal cells was verified with the analysis of brain sections from CDNF-deficient mice, where this IR pattern could also be observed (Fig. 5H)."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:28275710
Possibly Non-Specific
Extracted Antibody Information: "all used at 1:500 dilution. The localization pattern of infused rhCDNF in the striatum and cortex was confirmed with another anti-CDNF antibody derived from mouse (catalog #302-100, Icosagen AS; RRID: AB_11134475)."
Extracted Specificity Statement: "5A,E) nor in the TH-positive neurons of PBS-infused hemisphere (data not shown), although rare random background dots were sometimes visible throughout the sections. After chromogenic detection of anti-CDNF IR, all samples displayed cross-reactivity of the anti-CDNF antibody with an epitope in the nuclei of non-neuronal cells. The unspecific nature of this anti-CDNF IR in the nuclei of non-neuronal cells was verified with the analysis of brain sections from CDNF-deficient mice, where this IR pattern could also be observed (Fig."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:28275710
Source: Antibody Registry
