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MYPT1 (D6C1) Rabbit mAb antibody

RRID:AB_10998518

Antibody ID

AB_10998518

Target Antigen

MYPT1 (D6C1) Rabbit mAb h, mk, dg, human, non-human primate, canine

Proper Citation

(Cell Signaling Technology Cat# 8574, RRID:AB_10998518)

Clonality

monoclonal antibody

Reference

PMID:28486133

Comments

Applications: W, IP, IF-IC

Host Organism

rabbit

Vendor

Cell Signaling Technology

Cat Num

8574

Publications that use this research resource

RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6.

  • Durkin CH
  • Dev. Cell
  • 2017 May 8

Literature context:


Abstract:

RhoA-mediated regulation of myosin-II activity in the actin cortex controls the ability of cells to contract and bleb during a variety of cellular processes, including cell migration and division. Cell contraction and blebbing also frequently occur as part of the cytopathic effect seen during many different viral infections. We now demonstrate that the vaccinia virus protein F11, which localizes to the plasma membrane, is required for ROCK-mediated cell contraction from 2 hr post infection. Curiously, F11-induced cell contraction is dependent on RhoC and not RhoA signaling to ROCK. Moreover, RhoC-driven cell contraction depends on the upstream inhibition of RhoD signaling by F11. This inhibition prevents RhoD from regulating its downstream effector Pak6, alleviating the suppression of RhoC by the kinase. Our observations with vaccinia have now demonstrated that RhoD recruits Pak6 to the plasma membrane to antagonize RhoC signaling during cell contraction and blebbing.