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TOPORS (H-127) antibody

RRID:AB_10852342

Antibody ID

AB_10852342

Target Antigen

TOPORS (H-127) human, mouse, rat

Proper Citation

(Santa Cruz Biotechnology Cat# sc-292009, RRID:AB_10852342)

Clonality

monoclonal antibody

Reference

PMID:29499153

Comments

validation status unknown check with seller; recommendations: WB, IP, IF, ELISA; Immunofluorescence; ELISA; Immunoprecipitation; Western Blot

Host Organism

rabbit

Vendor

Santa Cruz Biotechnology

Cat Num

sc-292009

Publications that use this research resource

The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output.

  • Bulut-Karslioglu A
  • Cell Stem Cell
  • 2018 Mar 1

Literature context:


Abstract:

A permissive chromatin environment coupled to hypertranscription drives the rapid proliferation of embryonic stem cells (ESCs) and peri-implantation embryos. We carried out a genome-wide screen to systematically dissect the regulation of the euchromatic state of ESCs. The results revealed that cellular growth pathways, most prominently translation, perpetuate the euchromatic state and hypertranscription of ESCs. Acute inhibition of translation rapidly depletes euchromatic marks in mouse ESCs and blastocysts, concurrent with delocalization of RNA polymerase II and reduction in nascent transcription. Translation inhibition promotes rewiring of chromatin accessibility, which decreases at a subset of active developmental enhancers and increases at histone genes and transposable elements. Proteome-scale analyses revealed that several euchromatin regulators are unstable proteins and continuously depend on a high translational output. We propose that this mechanistic interdependence of euchromatin, transcription, and translation sets the pace of proliferation at peri-implantation and may be employed by other stem/progenitor cells.

Funding information:
  • NICHD NIH HHS - F30 HD093116()
  • NIGMS NIH HHS - R01 GM113014()
  • NIGMS NIH HHS - R01 GM123556()
  • NIGMS NIH HHS - R01 GM55040(United States)