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Phospho-cdc2 (Tyr15) Antibody

RRID:AB_10835695

Antibody ID

AB_331460

Target Antigen

Phospho-cdc2 (Tyr15) drosophila/arthropod, mouse, non-human primate, rat, human, h, m, r, mk, dm, x

Proper Citation

(Cell Signaling Technology Cat# 9111, RRID:AB_331460)

Clonality

polyclonal antibody

Comments

Applications: W, IP. Consolidation on 11/2018: AB_10104768, AB_10104769, AB_10835695, AB_2074655, AB_331460.

Host Organism

rabbit

Vendor

Cell Signaling Technology

Cat Num

9111

Publications that use this research resource

The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells.

  • Huang TH
  • Mol. Cell
  • 2018 Mar 1

Literature context:


Abstract:

The access-repair-restore model for the role of chromatin in DNA repair infers that chromatin is a mere obstacle to DNA repair. However, here we show that blocking chromatin assembly, via knockdown of the histone chaperones ASF1 or CAF-1 or a mutation that prevents ASF1A binding to histones, hinders Rad51 loading onto ssDNA during homologous recombination. This is a consequence of reduced recruitment of the Rad51 loader MMS22L-TONSL to ssDNA, resulting in persistent RPA foci, extensive DNA end resection, persistent activation of the ATR-Chk1 pathway, and cell cycle arrest. In agreement, histones occupy ssDNA during DNA repair in yeast. We also uncovered DNA-PKcs-dependent DNA damage-induced ASF1A phosphorylation, which enhances chromatin assembly, promoting MMS22L-TONSL recruitment and, hence, Rad51 loading. We propose that transient assembly of newly synthesized histones onto ssDNA serves to recruit MMS22L-TONSL to efficiently form the Rad51 nucleofilament for strand invasion, suggesting an active role of chromatin assembly in homologous recombination.

Funding information:
  • Intramural NIH HHS - (United States)
  • NCI NIH HHS - R01 CA095641()