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Neurokinin 1 Receptor Antibody

RRID:AB_10002802

Antibody ID

AB_10002802

Target Antigen

Neurokinin 1 Receptor guinea pig, human, rat

Proper Citation

(Novus Cat# NB300-119, RRID:AB_10002802)

Clonality

polyclonal antibody

Reference

PMID:29576387

Comments

Immunofluorescence, Flow cytometry / FACS analysis, Immunocytochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry, Western Blot

Host Organism

rabbit

Vendor

Novus

Cat Num

NB300-119

Publications that use this research resource

Functional Divergence of Delta and Mu Opioid Receptor Organization in CNS Pain Circuits.

  • Wang D
  • Neuron
  • 2018 Apr 4

Literature context:


Abstract:

Cellular interactions between delta and mu opioid receptors (DORs and MORs), including heteromerization, are thought to regulate opioid analgesia. However, the identity of the nociceptive neurons in which such interactions could occur in vivo remains elusive. Here we show that DOR-MOR co-expression is limited to small populations of excitatory interneurons and projection neurons in the spinal cord dorsal horn and unexpectedly predominates in ventral horn motor circuits. Similarly, DOR-MOR co-expression is rare in parabrachial, amygdalar, and cortical brain regions processing nociceptive information. We further demonstrate that in the discrete DOR-MOR co-expressing nociceptive neurons, the two receptors internalize and function independently. Finally, conditional knockout experiments revealed that DORs selectively regulate mechanical pain by controlling the excitability of somatostatin-positive dorsal horn interneurons. Collectively, our results illuminate the functional organization of DORs and MORs in CNS pain circuits and reappraise the importance of DOR-MOR cellular interactions for developing novel opioid analgesics.

Funding information:
  • NCI NIH HHS - P30 CA042014(United States)