AA2P-mediated DNA demethylation synergizes with stem cell agonists to promote expansion of hematopoietic stem cells.

Addgene (tool)

RRID:SCR_002037

Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.

View all literature mentions

PeproTech (tool)

RRID:SCR_006802

An Antibody supplier

View all literature mentions

FlowJo (tool)

RRID:SCR_008520

Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.

View all literature mentions

QIAGEN (tool)

RRID:SCR_008539

A commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.

View all literature mentions

Millipore (tool)

RRID:SCR_008983

An Antibody supplier

View all literature mentions

BD Biosciences (tool)

RRID:SCR_013311

An Antibody supplier

View all literature mentions

GENCODE (tool)

RRID:SCR_014966

Human and mouse genome annotation project which aims to identify all gene features in the human genome using computational analysis, manual annotation, and experimental validation.

View all literature mentions

Morpheus (tool)

RRID:SCR_014975

Modeling and simulation environment for study of multi scale and multicellular systems. Users can construct and simulate models of gene regulation, signaling pathways, tissue patterning and morphogenesis and explore the effects of multiscale feedbacks between these processes. Morpheus can render 2D and 3D models using graphical user interface.

View all literature mentions

DESeq2 (tool)

RRID:SCR_015687

Software package for differential gene expression analysis based on the negative binomial distribution. Used for analyzing RNA-seq data for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates.

View all literature mentions

Salmon (tool)

RRID:SCR_017036

Software tool for quantifying expression of transcripts using RNA-seq data. Provides fast and bias-aware quantification of transcript expression. Transcriptome-wide quantifier to correct for fragment GC-content bias.

View all literature mentions

Morpheus by Broad Institute (tool)

RRID:SCR_017386

Software tool for versatile matrix visualization and analysis. Program to generate heatmaps from input data. JavaScript matrix visualization and analysis.

View all literature mentions

ThermoFisher Scientific EVOS M5000 Imaging System (tool)

RRID:SCR_023650

Microsoft system includes integrated digital inverted benchtop microscope for four-color fluorescence, transmitted-light, and color imaging. Provides interchangeable optics with autofocus, single-click multi-channel image acquisition.3.2 Megapixels, monochrome, CMOS camera. Offers software for acquisition, analysis, and automated cell counting.

View all literature mentions

NSG-HLA-A2/HHD (NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(HLA-A/H2-D/B2M)1Dvs/SzJ) (tool)

RRID:BCBC_4611

NSG-HLA-A2/HHD mutant mice are immunodeficient and express human HLA class 1 heavy and light chains. This strain may be useful as a human hematopoietic engraftment host that supports the maturation of human T cells with transplantation http://jaxmice.jax.org/strain/014570.html.

View all literature mentions

GAS6 (D3A3G) Rabbit mAb (antibody)

RRID:AB_2799720

This monoclonal targets GAS6

View all literature mentions

H3K4me2-human (antibody)

RRID:AB_2560996

This polyclonal targets H3K4me2

View all literature mentions

Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb (PE Conjugate) (antibody)

RRID:AB_2798746

This monoclonal targets H3

View all literature mentions

Anti-5-methylcytosine, clone 33D3 (antibody)

RRID:AB_10863148

This monoclonal targets 5-methylcytosine clone 33D3

View all literature mentions

Rabbit Anti-Human Axl, phospho (tyr779) Affinity purified Polyclonal antibody, Unconjugated (antibody)

RRID:AB_2062560

This polyclonal targets Human Axl, phospho (Tyr779)

View all literature mentions

Goat Anti-Human Axl Polyclonal antibody, Unconjugated (antibody)

RRID:AB_354852

This polyclonal targets Human Axl

View all literature mentions

Human Gas6 Polyclonal Ab (antibody)

RRID:AB_354376

This polyclonal targets Human Gas6 Ab

View all literature mentions

PerCP/Cyanine5.5 anti-human CD10 (antibody)

RRID:AB_10642819

This monoclonal targets CD10

View all literature mentions

Mouse Anti-CD45RA Monoclonal Antibody, FITC Conjugated, Clone HI100 (antibody)

RRID:AB_395879

This monoclonal targets CD45RA

View all literature mentions

Mouse Anti-Human CD45RA Monoclonal Antibody, APC-H7 Conjugated, Clone HI100 (antibody)

RRID:AB_1727497

This monoclonal targets Human CD45RA

View all literature mentions

Mouse Anti-CD184 Monoclonal Antibody, Allophycocyanin Conjugated, Clone 12G5 (antibody)

RRID:AB_398616

This monoclonal targets CD184

View all literature mentions

APC anti-human CD201 (EPCR) (antibody)

RRID:AB_11126139

This monoclonal targets CD201

View all literature mentions

Mouse Anti-CD14 Monoclonal Antibody, Phycoerythrin Conjugated, Clone M5E2 (antibody)

RRID:AB_395799

This monoclonal targets CD14

View all literature mentions

Mouse Anti-Human CD123 Monoclonal Antibody, PerCP-Cy5.5 Conjugated, Clone 7G3 (antibody)

RRID:AB_1645547

This monoclonal targets CD123 (IL-3 Receptor α)

View all literature mentions

Mouse Anti-CD41a Monoclonal Antibody, Phycoerythrin Conjugated, Clone HIP8 (antibody)

RRID:AB_396624

This monoclonal targets CD41a

View all literature mentions

Mouse Anti-CD41a Monoclonal Antibody, FITC Conjugated, Clone HIP8 (antibody)

RRID:AB_395858

This monoclonal targets CD41a

View all literature mentions

Hu CD56 PE NCAM16.2 100 Tst (antibody)

RRID:AB_400017

This monoclonal targets CD56 (NCAM-1)

View all literature mentions

CD49f (antibody)

RRID:AB_11151910

This monoclonal targets CD49f (Integrin α6 chain)

View all literature mentions

CD90 (antibody)

RRID:AB_10714644

This monoclonal targets CD90

View all literature mentions

Mouse Anti-CD33 Monoclonal Antibody, Phycoerythrin Conjugated, Clone WM53 (antibody)

RRID:AB_395843

This monoclonal targets CD33

View all literature mentions

CD19 (antibody)

RRID:AB_395812

This monoclonal targets CD19

View all literature mentions

Mouse Anti-CD19 Monoclonal Antibody, Phycoerythrin Conjugated, Clone HIB19 (antibody)

RRID:AB_395813

This monoclonal targets CD19

View all literature mentions

Mouse Anti-CD3 Monoclonal Antibody, Allophycocyanin Conjugated, Clone UCHT1 (antibody)

RRID:AB_398591

This monoclonal targets CD3

View all literature mentions

CD45 (antibody)

RRID:AB_395874

This monoclonal targets CD45

View all literature mentions

Mouse Anti-CD45 Monoclonal Antibody, Allophycocyanin Conjugated, Clone HI30 (antibody)

RRID:AB_398600

This monoclonal targets CD45

View all literature mentions

Mouse Anti-CD38 Monoclonal Antibody, FITC Conjugated, Clone HIT2 (antibody)

RRID:AB_395852

This monoclonal targets CD38

View all literature mentions

CD38 (antibody)

RRID:AB_398599

This monoclonal targets CD38

View all literature mentions

PE Mouse Anti-Human CD34 Clone 581 (antibody)

RRID:AB_396151

This monoclonal targets CD34

View all literature mentions

GAS6 (D3A3G) Rabbit mAb (antibody)

RRID:AB_2799720

This monoclonal targets GAS6

View all literature mentions

GAS6 (D3A3G) Rabbit mAb (antibody)

RRID:AB_2799720

This monoclonal targets GAS6

View all literature mentions

H3K4me2-human (antibody)

RRID:AB_2560996

This polyclonal targets H3K4me2

View all literature mentions

H3K4me2-human (antibody)

RRID:AB_2560996

This polyclonal targets H3K4me2

View all literature mentions

Bio-Rad CFX96 Real-Time PCR Detection System (instrument resource)

RRID:SCR_018064

Real Time PCR detection system that combines optical technology with temperature control. Six channel, five colors and one FRET channel, real-time PCR instrument for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on integrated LCD touch screen, or use included CFX Maestro Software to design experiment and analyze results from connected computer.

View all literature mentions

Bio-Rad CFX96 Real-Time PCR Detection System (instrument resource)

RRID:SCR_018064

Real Time PCR detection system that combines optical technology with temperature control. Six channel, five colors and one FRET channel, real-time PCR instrument for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on integrated LCD touch screen, or use included CFX Maestro Software to design experiment and analyze results from connected computer.

View all literature mentions

Bio-Rad CFX96 Real-Time PCR Detection System (instrument resource)

RRID:SCR_018064

Real Time PCR detection system that combines optical technology with temperature control. Six channel, five colors and one FRET channel, real-time PCR instrument for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on integrated LCD touch screen, or use included CFX Maestro Software to design experiment and analyze results from connected computer.

View all literature mentions

Luminex Amnis ImageStreamX Mk II System (instrument resource)

RRID:SCR_018589

Bench top multispectral imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. It combines per cell information with statistical significance of sample sizes of standard flow cytometry.

View all literature mentions

Luminex Amnis ImageStreamX Mk II System (instrument resource)

RRID:SCR_018589

Bench top multispectral imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. It combines per cell information with statistical significance of sample sizes of standard flow cytometry.

View all literature mentions

Luminex Amnis ImageStreamX Mk II System (instrument resource)

RRID:SCR_018589

Bench top multispectral imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. It combines per cell information with statistical significance of sample sizes of standard flow cytometry.

View all literature mentions

Seurat (software resource)

RRID:SCR_016341

Software as R package designed for QC, analysis, and exploration of single cell RNA-seq data. Enable users to identify and interpret sources of heterogeneity from single cell transcriptomic measurements, and to integrate diverse types of single cell data.

View all literature mentions

Seurat (software resource)

RRID:SCR_016341

Software as R package designed for QC, analysis, and exploration of single cell RNA-seq data. Enable users to identify and interpret sources of heterogeneity from single cell transcriptomic measurements, and to integrate diverse types of single cell data.

View all literature mentions

Seurat (software resource)

RRID:SCR_016341

Software as R package designed for QC, analysis, and exploration of single cell RNA-seq data. Enable users to identify and interpret sources of heterogeneity from single cell transcriptomic measurements, and to integrate diverse types of single cell data.

View all literature mentions