X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Measuring trogocytosis between ovarian tumor and natural killer cells.

Antonio Delgado-Gonzalez | Ying-Wen Huang | Ermelinda Porpiglia | Kenyi Donoso | Veronica D Gonzalez | Wendy J Fantl
STAR protocols | 2022

Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes existing protocols while highlighting key experimental variables to demonstrate that natural killer (NK) cells acquire the tetraspanin CD9 by trogocytosis from ovarian tumor cells. For complete details on the use and execution of this protocol, please refer to Gonzalez et al. (2021).

Pubmed ID: 35693208 RIS Download

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


Anti-Human CD16 (3G8)-165Ho (antibody)

RRID:AB_2802109

This monoclonal targets CD16

View all literature mentions

Purified anti-human CD11c (antibody)

RRID:AB_314171

This monoclonal targets CD11c

View all literature mentions

CD9 (antibody)

RRID:AB_2741243

This monoclonal targets CD9

View all literature mentions

APC anti-human CD45 (antibody)

RRID:AB_2562049

This monoclonal targets CD45

View all literature mentions

CD9 antibody [MEM-61] (antibody)

RRID:AB_302894

This monoclonal targets CD9

View all literature mentions

Anti-PE (PE001)-165Ho Antibody (antibody)

RRID:AB_2714168

This monoclonal targets phycoerythrin

View all literature mentions

CD9 (antibody)

RRID:AB_395774

This monoclonal targets CD9

View all literature mentions

NK-92 (cell line)

RRID:CVCL_2142

Cell line NK-92 is a Cancer cell line with a species of origin Homo sapiens

View all literature mentions

NKL (cell line)

RRID:CVCL_0466

Cell line NKL is a Cancer cell line with a species of origin Homo sapiens (Human)

View all literature mentions