URL: http://www.addgene.org/98290
Proper Citation: RRID:Addgene_98290
Insert Name: P2A-puro
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30894629
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Backbone Size:14877; Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: P2A-antibiotic resistance gene cassettes from the plasmids in this deposit (https://www.addgene.org/depositing/74286/) can be cloned in place of the P2A-puro gene using the unique BamHI and MluI sites. The second MluI site in the original lentiCRISPRv2 plasmid was destroyed by Klenow end-filling and religation after MluI digestion. For target guide (sgRNA) sequence cloning instructions, please see https://www.addgene.org/52961 for the Zhang's lab lentiCRISPR v2 guide.
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Source: Addgene