URL: http://www.addgene.org/8732
Proper Citation: RRID:Addgene_8732
Insert Name: yECitrine
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15197731
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Full name of this plasmid is pFA6a-link-yECitrine-KanR. yECitrine is created by mutagenesis from a codon-optimized green fluorescent protein - yEGFP1 (Cormack et al. 1997). This yEGFP variant is cloned into pDH5, replacing YFP. An codon-optimized linker was added into the yEGFP variant to improve expression level. The G418 resistance marker (KanR) was introduced into the plasmid by subcloning the BglII/EcoRI fragment of pDH3 (Hailey et al., 2002) into the plasmid, in place of SpHIS5. The yeast GFP used to create this plasmid contained a point mutation M233I that is present in all GFP variants derived from this original yEGFP. The mutation had no effect on fluorescence (see associated publication for more details).
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Source: Addgene