URL: http://www.addgene.org/74293
Proper Citation: RRID:Addgene_74293
Insert Name: LacZ-alpha
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27089539
Vector Backbone Description: Backbone Marker:Genecopedia; Backbone Size:10415; Vector Backbone:pCRISPR-CG01; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: See Supporting information file 1: "Instructions for one-step gRNA cloning into All-in-one CRISPR/Cas9 LacZ" from associated publication for gRNA cloning instructions using this plasmid. Please cite associated publication when using this plasmid for cloning.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org