URL: http://www.addgene.org/52963
Proper Citation: RRID:Addgene_52963
Insert Name: S. pyogenes sgRNA cassette
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25075903
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information.
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Source: Addgene