URL: http://www.addgene.org/46266
Proper Citation: RRID:Addgene_46266
Insert Name: Vcl T12 mutant
Organism: Gallus gallus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:15728584
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: T12 mutation introduced by QuikChange PCR to Addgene plasmid 46265. Mutant inhibits vinculin autoinhibition by ~ 100 fold, vinculin is partially activated and will bind talin, but not actin (unless both talin and actin are present at same time).
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No rating or validation information has been found for pEGFPC1/GgVcl 1-1066 T12 mutant.
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Source: Addgene