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Plasmid Name
RRID:Addgene_45597 RRID Copied  
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RRID:Addgene_45597
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Plasmid Information

URL: http://www.addgene.org/45597

Proper Citation: RRID:Addgene_45597

Insert Name: c-Myc

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:15048125

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: Alternate plasmid names: pD40-His-c-Myc; pcDNA-DEST40 Myc wt This His6-tagged c-Myc plasmid was created by PCR amplification of the coding sequence for wild-type murine c-Myc2 from the CMVMyc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-Myc. For plasmid usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971).

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Data and Source Information

Source: Addgene