URL: http://www.addgene.org/41596
Proper Citation: RRID:Addgene_41596
Insert Name: His3
Organism: S. kluyveri
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9717241
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Comments: Please note that the associated publication contains important primer information for the intended application of this plasmid, but refers to a version of the plasmid containing the S. pombe HIS5 gene while this plasmid contains the S. kluyveri HIS3 gene. The generation of both plasmids is described in Wach, A., Brachat, A., Alberti-Segui, C., Rebischung, C. and Philippsen, P. (1997). Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast 13, 1065–1075. Pubmed ID: 9290211.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org