URL: http://www.addgene.org/32480
Proper Citation: RRID:Addgene_32480
Insert Name: PSAML141F,Y115F:GlyR
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21885782
Vector Backbone Description: Backbone Size:7200; Vector Backbone:pCAGGS- IRES-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Note on the difference between PSAMY115F,L141F:GlyR and PSAML141F:GlyR silencers: In Magnus et al, the PSAMY115F,L141F-GlyR channel was emphasized for silencing because this channel has the lowest acetylcholine responsiveness. However, PSAML141F-GlyR constructs, lacking the Y115F mutation, already have low ACh potency. The Y115F mutation also slightly increases the EC50 for the activator ligand, PSEM89S. Since PSAML141F-GlyR constructs already have very low acetylcholine potency, in many cases it may not be worth the slight reduction in PSEM potency. We have made both constructs available for researchers who may find that they have different requirements with respect to acetylcholine responsiveness.
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No rating or validation information has been found for CAG::PSAML141F,Y115F:GlyR-IRES-GFP.
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Source: Addgene