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Plasmid Name
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RRID:Addgene_31057
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Plasmid Information

URL: http://www.addgene.org/31057

Proper Citation: RRID:Addgene_31057

Insert Name: Ankyrin B

Organism: Homo sapiens

Bacterial Resistance: Kanamycin

Defining Citation: PMID:21186323

Vector Backbone Description: Backbone Size:4000; Vector Backbone:Modified pEGFP-N1 (see note); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin

Comments: HA-ankyrin-B was prepared based on an ankyrin-B-GFP template in a pEGFP-N1 vector (Mohler et al., 2002). GFP was cut out between the PmeI and the NotI sites and replaced with a 2XHA-tag encoding sequence (YPYDVPDYA) with a PmeI site on its 5′ end and a stop codon followed by a NotI site on the 3′ end that was synthesized by PCR.

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Data and Source Information

Source: Addgene