URL: http://www.addgene.org/26536
Proper Citation: RRID:Addgene_26536
Bacterial Resistance: Chloramphenicol and Kanamycin
Defining Citation: PMID:20967238
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:Other, T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: For cloning taq polymerase-amplified PCR products, digest vector with Eam1105I, purify, ligate with PCR product, and use to transform DH5-alpha cells (or strain of choice). dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Expand AllWe found {{ ctrl2.mentions.total_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
Find mentions based on location
{{ ctrl2.mentions.errors.location }}
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pJC53.2.
No alerts have been found for pJC53.2.
Source: Addgene