URL: http://www.addgene.org/21074
Proper Citation: RRID:Addgene_21074
Insert Name: microtubule-associated protein 1 light chain 3 beta
Organism: Rattus norvegicus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:17534139
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pEGFP-C1+mRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82 NOTE FROM AUTHORS: We found several alterations between our plasmid and the version on NCBI [Map1lc3b; https://www.ncbi.nlm.nih.gov/gene/64862) ]. Our original clone may be a kind of variant. In the C-terminal end of tfLC3, the stop codon is removed, which causes an additional 17 amino acid residues. However, the C-terminal end from Gly120, including the 17 amino acids, is immediately cut off by Atg4 protease after the translation. Therefore that sequence variant will not affect the function of the protein.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org