URL: http://www.addgene.org/21074
Proper Citation: RRID:Addgene_21074
Insert Name: microtubule-associated protein 1 light chain 3 beta
Organism: Rattus norvegicus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:17534139
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pEGFP-C1+mRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82 NOTE FROM AUTHORS: We found several alterations between our plasmid and the version on NCBI [Map1lc3b; https://www.ncbi.nlm.nih.gov/gene/64862) ]. Our original clone may be a kind of variant. In the C-terminal end of tfLC3, the stop codon is removed, which causes an additional 17 amino acid residues. However, the C-terminal end from Gly120, including the 17 amino acids, is immediately cut off by Atg4 protease after the translation. Therefore that sequence variant will not affect the function of the protein.
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Source: Addgene