URL: http://www.addgene.org/20927
Proper Citation: RRID:Addgene_20927
Insert Name: Oct4 and Sox2; SV40LT and Klf4
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19325077
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10600; Vector Backbone:pCEP4; Vector Types:Episomal/EBNA; Bacterial Resistance:Ampicillin
Comments: Please note Addgene Quality Control sequence found mutation K278T in KLF4. Oct4 and Sox2 are coexpressed under a strong EF promoter. SV40LT and Klf4 are coexpressed under a separate (but identical) EF promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells. These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul. Addgene recommends screening 3-5 colonies immediately upon receipt, as this plasmid may be prone to recombination.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org