URL: http://www.addgene.org/18917
Proper Citation: RRID:Addgene_18917
Insert Name: Channelrhodopsin 2-tdtomato
Organism: Chlamydomonas reinhardtii
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18614669
Vector Backbone Description: Backbone Size:5340; Vector Backbone:AAV2; Vector Types:AAV, Cre/Lox, Other, Adeno-Associated Virus; Bacterial Resistance:Ampicillin
Comments: These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in Stbl2 cells from Invitrogen. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed. The expected patterns can be calculated from the attached sequence. Please see Reviews in the right column for an image of Addgene's digest with these enzymes.
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Source: Addgene