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Plasmid Name
RRID:Addgene_1864 RRID Copied  
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RRID:Addgene_1864
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Plasmid Information

URL: http://www.addgene.org/1864

Proper Citation: RRID:Addgene_1864

Insert Name: scramble

Bacterial Resistance: Ampicillin

Defining Citation: PMID:15718470

Vector Backbone Description: Backbone Marker:Stewart SA, RNA 2003 Apr; 9(4):493-501.; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin

Comments: shRNA for use as a negative control. Sequence of hairpin is:CCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGG. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454). Please note that the 5' cloning site, AgeI, is typically destroyed during the shRNA cloning. Depending on the specific shRNA sequence, the site can occasionally be restored. AgeI is present in this plasmid.

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Ratings and Alerts

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Data and Source Information

Source: Addgene