URL: http://www.addgene.org/17408
Proper Citation: RRID:Addgene_17408
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18308936
Vector Backbone Description: Backbone Marker:Jacks Lab; Backbone Size:9200; Vector Backbone:Puro.Cre; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Puro.Cre was initially generated by amplification of PuroR from MSCV.Puro (Clontech) with PuroF (5’-CGCTGCGCCGAATTCATGACCGAGTACAAGCCCACG-3’) and PuroR (5’-TAAGCGGCCGCTCGAGTCAGGCACCGGGCTTGCGGGTC-3’), digestion with EcoRI and NotI and cloning into UbC.Luciferase.PGK.Cre to generate Puro.Cre. For lentiviral packaging, you can use pCMV-delta-8.2 (Addgene #8455) and pCMV-VSV-G (Addgene #8454).
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Source: Addgene