URL: http://www.addgene.org/163642
Proper Citation: RRID:Addgene_163642
Insert Name: DHB::GFP-P2A-H2B::2xmKate2::3xHA
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:33350383
Vector Backbone Description: Backbone Marker:Bob Goldstein/Ari Pani; Backbone Size:11401; Vector Backbone:pAP088; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: GFP fusion of C. elegans codon-optimized DNA Helicase B (DHB) under the rps-27 promoter for ubiquitous expression, co-expressed with H2B::2xmKate2. Plasmid is for CRISPR/Cas9-mediated single copy insertion at the ttTi4348 site on Chromosome I and can be paired with sgRNA plasmid pAP082 Please note: Plasmid contains a 117bp deletion in left homology arm relative to the depositor's provided sequence. This deletion is not known to affect plasmid function, but may slightly lower knock-in efficiency.
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Source: Addgene