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Plasmid Name
RRID:Addgene_163640 RRID Copied  
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RRID:Addgene_163640
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Plasmid Information

URL: http://www.addgene.org/163640

Proper Citation: RRID:Addgene_163640

Insert Name: DHB::2mKate2-P2A-H2B::GFP::3xHA

Organism: Caenorhabditis elegans

Bacterial Resistance: Ampicillin

Defining Citation: PMID:33350383

Vector Backbone Description: Backbone Marker:Bob Goldstein/Ari Pani; Backbone Size:11926; Vector Backbone:pAP088; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin

Comments: 2xmKate2 fusion of C. elegans codon-optimized DNA Helicase B (DHB) under the rps-27 promoter for ubiquitous expression, co-expressed with H2B::GFP. Plasmid is for CRISPR/Cas9-mediated single copy insertion at the ttTi4348 site on Chromosome I and can be paired with sgRNA plasmid pAP082

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Data and Source Information

Source: Addgene