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Plasmid Name
RRID:Addgene_14695 RRID Copied  
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RRID:Addgene_14695
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Plasmid Information

URL: http://www.addgene.org/14695

Proper Citation: RRID:Addgene_14695

Insert Name: BARK1 minigene

Organism: Bos taurus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:8119963

Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: The cDNA encoding bovine BARK1 residues Gly495 to Leu689 was amplified using PCR to construct a minigene casette with newly engineered 5' and 3' ends (see Author's map). The 5' end contained an EcoRI site for subcloning, followed by the kozak sequence 5'-GCCGCCACCATGG-3'. A bclI site was inserted at the 3' end immediately following the stop codon. The final BARK1 minigene construct contains the 3'UTR of the human beta-globin gene and a polyadenylation signal.

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Data and Source Information

Source: Addgene