You are being redirected to the external resource authority website. If you haven't been redirected in 10 seconds, please click http://www.addgene.org/12258.

For additional information about this resource, such as mentions, alerts, rating and validation information, please view our Resource Report (shown below).


Plasmid Name
RRID:Addgene_12258 RRID Copied  
PDF Report How to cite
RRID:Addgene_12258
Copy Citation Copied
Plasmid Information

URL: http://www.addgene.org/12258

Proper Citation: RRID:Addgene_12258

Bacterial Resistance: Ampicillin

Defining Citation: PMID:

Vector Backbone Description: Backbone Size:10455; Vector Backbone:pWPXLd; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin

Comments: pWPXLd is essentially pWPXL without the LoxP site in the 3' LTR. pWPT or pWPXL can be used for constitutive transgene expression. pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter Unique restriction sites at key positions will allow you to change promoter and transgene. PmeI is a popular site for cloning. You can also use PacI and SwaI. Please note that ClaI in these vectors is blocked by Dam methylation. The plasmids need to be grown in a Dam- bacteria strain, if you wish to use ClaI for cloning. Packaging plasmids for Trono lab lentiviral vectors are also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. More accurate sequence can be found in the supplement documents ("Notes from Addgene") above. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.

Expand All
Usage and Citation Metrics
We apologize, the data for 2022 is currently unavailable for most resources. We are aware of the issue and are working to resolve it.

We found {{ ctrl2.mentions.total_count }} mentions in open access literature.

We have not found any literature mentions for this resource.

We are searching literature mentions for this resource.

View full usage report

Most recent articles:

{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})

Checkfor all resource mentions.

Collaborator Network

A list of researchers who have used the resource and an author search tool

Find mentions based on location


{{ ctrl2.mentions.errors.location }}

A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.

Ratings and Alerts

No rating or validation information has been found for pWPXLd.

No alerts have been found for pWPXLd.

Data and Source Information

Source: Addgene