URL: http://www.addgene.org/12258
Proper Citation: RRID:Addgene_12258
Bacterial Resistance: Ampicillin
Defining Citation: PMID:
Vector Backbone Description: Backbone Size:10455; Vector Backbone:pWPXLd; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: pWPXLd is essentially pWPXL without the LoxP site in the 3' LTR. pWPT or pWPXL can be used for constitutive transgene expression. pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter Unique restriction sites at key positions will allow you to change promoter and transgene. PmeI is a popular site for cloning. You can also use PacI and SwaI. Please note that ClaI in these vectors is blocked by Dam methylation. The plasmids need to be grown in a Dam- bacteria strain, if you wish to use ClaI for cloning. Packaging plasmids for Trono lab lentiviral vectors are also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. More accurate sequence can be found in the supplement documents ("Notes from Addgene") above. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.
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Source: Addgene