URL: http://www.addgene.org/12245
Proper Citation: RRID:Addgene_12245
Insert Name: TERT, HSV1-TK
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11020357
Vector Backbone Description: Backbone Size:13115; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: CMV promoter is upstream of the bicistronic coding cassette with hTert and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
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Source: Addgene