X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

GAPDH antibody

RRID:AB_732652

Antibody ID

AB_732652

Target Antigen

GAPDH antibody human, mouse, rat, baboon, mouse, non-human primate, rat, human

Vendor

Abcam

Cat Num

ab37168

Proper Citation

(Abcam Cat# ab37168, RRID:AB_732652)

Reference

PMID:29932899

Clonality

polyclonal antibody

Host Organism

rabbit

Comments

seller recommendations: Immunohistochemistry; Western Blot; Immunohistochemistry - fixed; Immunohistochemistry - frozen; Immunofluorescence; Immunocytochemistry; ICC/IF, IHC-Fr, IHC-P, WB

Publications that use this research resource

Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation.

  • Yamazaki T
  • Mol. Cell
  • 2018 Jun 21

Literature context: t polyclonal anti-GAPDHAbcamCat#ab37168Mouse monoclonal anti-Digoxigeni


Abstract:

A class of long noncoding RNAs (lncRNAs) has architectural functions in nuclear body construction; however, specific RNA domains dictating their architectural functions remain uninvestigated. Here, we identified the domains of the architectural NEAT1 lncRNA that construct paraspeckles. Systematic deletion of NEAT1 portions using CRISPR/Cas9 in haploid cells revealed modular domains of NEAT1 important for RNA stability, isoform switching, and paraspeckle assembly. The middle domain, containing functionally redundant subdomains, was responsible for paraspeckle assembly. Artificial tethering of the NONO protein to a NEAT1_2 mutant lacking the functional subdomains rescued paraspeckle assembly, and this required the NOPS dimerization domain of NONO. Paraspeckles exhibit phase-separated properties including susceptibility to 1,6-hexanediol treatment. RNA fragments of the NEAT1_2 subdomains preferentially bound NONO/SFPQ, leading to phase-separated aggregates in vitro. Thus, we demonstrate that the enrichment of NONO dimers on the redundant NEAT1_2 subdomains initiates construction of phase-separated paraspeckles, providing mechanistic insights into lncRNA-based nuclear body formation.

Funding information:
  • NIAID NIH HHS - R01 AI050113(United States)