URL: http://www.addgene.org/80033
Proper Citation: RRID:Addgene_80033
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26657635
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2892; Vector Backbone:pAAV-MCS; Vector Types:AAV, Cre/Lox, a platform vector to construct targeting vectors by inserting 5' and 3' arms; Bacterial Resistance:Ampicillin
Comments: Upon construction of a targeting vector with pAAV-2Aneo v2, the “frame adjuster” should be cleaved with BspEI or BsrGI and then self-religated, or left untreated, so that 2Aneo is translated in frame with the 5' portion of the targeted endogenous gene. This truncation of the frame adjuster should be done prior to the incorporation of 5' and 3' arms into pAAV-2Aneo v2, if arms carry restriction enzyme sites cleaved during the truncation of the frame adjuster (i.e., BspEI or BsrGI sites). This plasmid and pAAV-2Aneo differ only by the sequences of the frame adjusters. A following article describes how to use pAAV-2Aneo v2 to construct AAV-based targeting vectors: Karnan et al. Bio-Protoc, 6(24): e2058, 2016. (http://www.bio-protocol.org/e2058)
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Source: Addgene