URL: http://www.addgene.org/78742
Proper Citation: RRID:Addgene_78742
Insert Name: LbCpf1 crRNA backbone, without spacer sequence
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27347757
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Use BsmBI sites to insert crRNA spacer sequence into the vector. This plasmid will express gRNA in mammalian cells for Cpf1 from Lachnospiraceae bacterium ND2006. Recommended to use with SQT1665 (Addgene plasmid# 78744), which expresses Lachnospiraceae bacterium ND2006 Cpf1. Please visit https://www.biorxiv.org/content/early/2016/06/09/057802 for bioRxiv preprint.
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Source: Addgene