URL: http://www.addgene.org/78741
Proper Citation: RRID:Addgene_78741
Insert Name: AsCpf1 crRNA backbone, without spacer sequence
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27347757
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Use BsmBI sites to insert crRNA spacer sequence into the vector. This plasmid will express gRNA in mammalian cells for Cpf1 from Acidaminococcus sp. (strain BV3L6). Recommended to use with SQT1659 (Addgene plasmid# 78743), which expresses Acidaminococcus sp. Cpf1. Please visit https://www.biorxiv.org/content/early/2016/06/09/057802 for bioRxiv preprint.
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Source: Addgene