URL: http://www.addgene.org/78311
Proper Citation: RRID:Addgene_78311
Insert Name: WT SpCas9-T2A-EGFP-ires-puromycin resistance
Organism: Streptococcus pyogenes
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28402852
Vector Backbone Description: Vector Backbone:PyCAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Cas9 expressing vector for genome editing of human pluripotent stem cells. Transfectants can be selected by EGFP+ sorting or transient treatment with puromycin. Described in Saarimäki-Vire, Balboa et al- 2016.
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Source: Addgene