URL: http://www.addgene.org/12525
Proper Citation: RRID:Addgene_12525
Insert Name: PIK3CA E545K
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16339315
Vector Backbone Description: Backbone Marker:Available at Addgene (plasmid #1764); Backbone Size:5150; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Mutation generated using Strategene Quickchange site-directed mutagenesis kit. See article for primer information. Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val (I143V when compared to NP_006209.2). It could be a SNP. No functional changes have been observed so far in signaling assays.
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Source: Addgene