URL: http://www.addgene.org/12172
Proper Citation: RRID:Addgene_12172
Insert Name: NpuDnaE
Organism: Nostoc punctiforme
Bacterial Resistance: Kanamycin
Defining Citation: PMID:16516207
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3757; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: pSKDuet01 contains two NdeI sites in the plasmid. To clone a gene of interest between NdeI and BamHI for constructing a fusion protein with NpuDnaE N-intein, please use pHYRSF1 (http://www.addgene.org/34549/), which lacks the second T7 promoter and NdeI site.
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Source: Addgene