URL: http://www.addgene.org/117986
Proper Citation: RRID:Addgene_117986
Insert Name: sgRNA backbone (F+E)
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30580963
Vector Backbone Description: Vector Backbone:pCESAx (Addgene 59986); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Users should follow the oligo cloning protocol for plasmid lentiCRISPR v2 (Addgene #52961) to 1) remove stuffer fragment and 2) insert annealed oligos to complete gRNA.
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Source: Addgene