Cervical (C5), unilateral spinal cord injury with diverse injury modalities, multiple behavioral outcomes, and histopathology

DOI:10.7295/W9T72FMZ

DATASET CITATION

Ferguson, A.R., Irvine,K.-A., Gensel, J.C., Nielson, J.L., Lin, A., Ly, J., Segal, M.R., Ratan, R.R., Bresnahan, J.C., Beattie, M.S. (2018) Cervical (C5), unilateral spinal cord injury with diverse injury modalities, multiple behavioral outcomes, and histopathology. Open Data Commons for Spinal Cord Injury. ODC-SCI:26 http://doi.org/10.7295/W9T72FMZ

ABSTRACT

STUDY PURPOSE: This project was a multivariate validation study of unilateral cervical contusion (hemi-contusion) model-development efforts.

DATA COLLECTED: The dataset includes N=159 rats with hemisections (n=9), NYU MASCIS weight drop contusions: sham (n=10), 6.25 mm (n=10), 12.5 mm (n=32); Infinite Horizon Impactor: sham (n=6), 75 kdyn (n=58), 100 kdyn (n=34). Behavioral recovery was monitored over 6 weeks using mutiple assements taken from the same subjects: Grooming Score, Paw Placement in a Cylinder, BBB locomotor subscore, Forelimb Open Field Score, and numerous Catwalk footprint analysis metrics. Histopathology metrics included lesion epicenter gray matter sparing, white matter sparing, total tissue sparing, and motorneuron numbers.

PRIMARY CONCLUSION: Principal component analysis (PCA)-based multidimensional pattern detectors can resolve the ‘syndrome space’ across multiple models of SCI, allowing direct comparison of subjects with diverse injury types, outcome sets and therapeutics.

KEYWORDS

bioinformatics; rodents; syndromics; translation

PROVENANCE / ORIGINATING PUBLICATIONS

• Ferguson, A. R., Irvine, K.-A., Gensel, J. C., Nielson, J. L., Lin, A., Ly, J., … Beattie, M. S. (2013). Derivation of Multivariate Syndromic Outcome Metrics for Consistent Testing across Multiple Models of Cervical Spinal Cord Injury in Rats. PLoS ONE, 8(3), e59712. doi:10.1371/journal.pone.0059712.

Primary data collected as part of this paper included model development efforts for the Infinite Horizon (force driven) injury data collected in the course of developing the unilateral contusion model in the rat. Analysis of these data are reported in primary form as part of this paper and then analyzed in ensemble with NYU/MASCIS data reused from Gensel et al. 2006 as an analytical derivative work product. These data were reused in combination with other data for larger scale analysis in Nielson et al., 2015.

• Nielson, J. L., Paquette, J., Liu, A. W., Guandique, C. F., Tovar, C. A., Inoue, T., … Ferguson, A. R. (2015). Topological data analysis for discovery in preclinical spinal cord injury and traumatic brain injury. Nature Communications, 6(1). doi:10.1038/ncomms9581.

This paper represented a purely derivative works paper that involved large scale analysis of data cross curated from Gensel et al. 2006; Ferguson et al., 2013, along with other data sources.

• Gensel, J. "Modeling and treatment of rat cervical spinal cord injury." Electronic Thesis or Dissertation. Ohio State University, 2007. https://etd.ohiolink.edu/

Primary data collected as part of this dissertation represented the full set of NYU/MASCIS (weight drop) injury data collected in the course of developing the unilateral contusion model in the rat. Analysis of these data were subsequently reported partially in Gensel et al., 2006, with the remaining data published as part of large scale analysis in combination with other data in Ferguson et al., 2013 and Nielson et al., 2015.

• Gensel, J. C., Tovar, C. A., Hamers, F. P. T., Deibert, R. J., Beattie, M. S., & Bresnahan, J. C. (2006). Behavioral and Histological Characterization of Unilateral Cervical Spinal Cord Contusion Injury in Rats. Journal of Neurotrauma, 23(1), 36–54. doi:10.1089/neu.2006.23.36

This paper reports the primary data on the development of the unilateral contusion spinal cord injury model in rats using the NYU/MASCIS device. The reported outcomes include 6 weeks of behavioral data collection using a number of endpoints collected on the same animals as well as detailed histology. This represents a peer-reviewed and published subset of the data collected as part of the Gensel dissertation. These data were included, along with additional curated data in Ferguson et al., 2013 and Nielson et al., 2015.

DATASET INFO

Contact: Adam Ferguson (Adam.Ferguson@ucsf.edu)

Lab: Ferguson Lab

ODC-SCI Accession: 26

Records in Dataset: 159

Fields per Record: 119

Files: 2

LICENSE

Creative Commons Attribution License (CC-BY 4.0)

FUNDING AND ACKNOWLEDGEMENTS

National Institutes of Health grants NS067092 (ARF), NS069537 (ARF), NS038079 (JCB and MSB), NS031193 (MSB and JCB), AG032518 (MSB and JCB), F32NS079030 (JLN), NS079030 (JLN), NS032000 (WY; MSB site PI, multicentre study); NYSCoRE CO19772 (MSB and JCB); the Department of Defense (DoD) grants W81XWH-10-1-0910 (MSB) and W81XWH-13-1-0297 (MSB); Craig H. Neilsen Foundation Grant 224308 (ARF); International Spinal Research Trust (MSB JCB); Wings for Life Foundation grant WFLUS008/12 (ARF) and WFLUS006/14 (ARF)

CONTRIBUTORS

Ferguson, Adam R. [ORCID:0000-0001-7102-1608]

University of California San Francisco (UCSF)

Irvine, Karen-Amanda [ORCID:0000-0001-5259-1824]

University of California San Francisco (UCSF)

Gensel, John C. [ORCID0000-0001-8980-108X]

University of California San Francisco (UCSF)

Nielson, Jessica L. [ORCID:0000-0002-3677-3959]

University of California San Francisco (UCSF)

Lin, Amity

University of California San Francisco (UCSF)

Ly, Johnathan

University of California San Francisco (UCSF)

Segal, Mark R.

University of California San Francisco (UCSF)

Ratan, Rajiv R.

Burke Rehabilitation Institute, Weill Cornell Medical College

Bresnahan, Jacqueline C. [ORCID:0000-0003-2243-7054]

University of California San Francisco (UCSF)

Beattie, Michael S. [ORCID:0000-0001-9463-3631]

University of California San Francisco (UCSF)

Tovar, Amy C.

The Ohio State University

Hamers, Frank P.T.

University of Utrecht, The Netherlands

Deibert, Rochelle J.

The Ohio State University

Van Meter, Montina

The Ohio State University

Komon, John

The Ohio State University

Forrider, Crystal

The Ohio State University

Huie, J. Russell [ORCID: 0000-0001-5594-4277]

University of California San Francisco (UCSF)

Inoue, Tomoo

University of California San Francisco (UCSF)

Nout, Yvette S. [ORCID: 0000-0001-6583-2308]

University of California San Francisco (UCSF)

Stuck, Ellen D.

University of California San Francisco (UCSF)

Talbott,Jason F. [ORCID: 0000-0003-3943-5530]

University of California San Francisco (UCSF)

Almeida, Carlos [ORCID: 0000-0001-9997-8719]

University of California San Francisco (UCSF)