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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00037674
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020284(mel-46)
Genomic Alteration: WBGene00020284(mel-46)
Availability: available
References:
Synonyms: mel-46(ok3760) IV.
Alternate IDs: WB-STRAIN:VC3108, CGC_VC3108
Notes: Made_by: Vancouver KO Group|"T06A10.1. External left primer: CAGCTTGTCTCCCGAATCTC. External right primer: AGGCCAACAATAGCCAAAAA. Internal left primer: CTCGTCTTTCTCGCGTTTTC. Internal right primer: TTTGAGCAATTCTGGACTAAAAA. Internal WT amplicon: 1270 bp. Deletion size: 448 bp. Deletion left flank: GACGTGAAGGCTTCACGAATGTGTTGGAGC. Deletion right flank: ACAGAAAAATGGGCGGGGCACAGTTTTGCA. Insertion Sequence: AGAAAAAT."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037674 Copy
http://www.wormbase.org/db/get?name=WBStrain00037677
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00016558(pks-1)
Genomic Alteration: WBGene00016558(pks-1)
Availability: available
References:
Synonyms: C41A3.1(ok3769) X.
Alternate IDs: WB-STRAIN:VC3112, CGC_VC3112
Notes: C41A3.1. External left primer: AAGCTTGGCGATCAGGTAGA. External right primer: CAGTTGACTCAATTTCCGCA. Internal left primer: ACGGCATAATACCGAACCAG. Internal right primer: TGCTCGTCAACAATGTTCGT. Internal WT amplicon: 1141 bp. Deletion size: 689 bp. Deletion left flank: CTCAATCCGACTCTGCGATGGAGGATATTT. Deletion right flank: GATCTGCCAGCTATTTGCTTGTGGGTTTGA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037677 Copy
http://www.wormbase.org/db/get?name=WBStrain00037683
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006815(unc-83)|WBGene00011581(T07D10.1)|WBGene00013786(nep-24)|WBGene00019119(F59E12.3)
Genomic Alteration: WBGene00006815(unc-83), WBGene00011581(T07D10.1), WBGene00013786(nep-24), WBGene00019119(F59E12.3)
Availability: available
References:
Synonyms: T07D10.1(gk3249) I; F59E12.3(gk3183) II; Y116A8C.5(gk3250) IV; unc-83(gk3251) gkDf35 V; gkDf32 X.
Alternate IDs: WB-STRAIN:VC3121, CGC_VC3121
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain is homozygous for a deletion (gk3183) in F59E12.3, detectable by PCR using the following primers. External left primer: GCATGCAAGAAATGCAAGAA. External right primer: TGAAGTCGCGCACAAATAAG. Internal left primer: TCACAAATGGAAACGTGTGG. Internal right primer: CAACGAGGCCAAAGTGATTT. Internal WT amplicon: 1320 bp. Deletion size: 585 bp. Deletion left flank: GAACTGACAACAAGTATCTCAACCTACACG. Deletion right flank: CCCCCGTTTATGCGCCCAGGGCATCCCACA. Validation: gk3183 passed by CGH. Other deletions (gkDf32, gkDf35, gk3249, gk3250, gk3251) identified by CGH."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037683 Copy
http://www.wormbase.org/db/get?name=WBStrain00037681
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003056(lon-2)|WBGene00010870(let-522)
Genomic Alteration: WBGene00003056(lon-2), WBGene00010870(let-522)
Availability: available
References:
Synonyms: M05B5.2(ok3716)/szT1 [lon-2(e678)] I; +/szT1 X.
Alternate IDs: WB-STRAIN:VC3118, CGC_VC3118
Notes: M05B5.2. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3716 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: AGGCAGTTTCAGGGTTCAAA. External right primer: CTAAGGCACTTGGCTTTTGC. Internal left primer: GGGAGGAAATTTCAAAAATGA. Internal right primer: AAAAATTTAACGCGTCGCTG. Internal WT amplicon: 1169 bp. Deletion size: 569 bp. Deletion left flank: GGAATGGCAAATTGACAGCATGAGGGTTTC. Deletion right flank: TTTTTGGGATGTTCAGCGACGCGTTAAATT. Insertion Sequence: TTT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037681 Copy
http://www.wormbase.org/db/get?name=WBStrain00037648
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00009925(F52B11.2)
Genomic Alteration: WBGene00009925(F52B11.2)
Availability: available
References:
Synonyms: F52B11.2(ok3718) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC3054, CGC_VC3054
Notes: F52B11.2. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3718 homozygotes (early- to mid-larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GTCCTGAAATATGGCGGAGA. External right primer: TCTTCTGGCCCTTCAACAGT. Internal left primer: ACACGAAGCACTGGCTTTTT. Internal right primer: GTCCGACAGTCCGTTCGT. Internal WT amplicon: 1267 bp. Deletion size: 518 bp. Deletion left flank: AATGTATTATTTTCCATTTTCCGAATTTTT. Deletion right flank: CGGATTCAAGGGCACCGAACCGTATCCAGT. Insertion Sequence: TT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037648 Copy
http://www.wormbase.org/db/get?name=WBStrain00037642
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00007357(C06A12.3)
Genomic Alteration: WBGene00007357(C06A12.3)
Availability: available
References:
Synonyms: C06A12.3(ok3746) IV.
Alternate IDs: WB-STRAIN:VC3042, CGC_VC3042
Notes: C06A12.3. External left primer: CAATGCAACGCCAATTGTTA. External right primer: CTCATCAATGCCTTGCTCCT. Internal left primer: TCCATTGTTTGAAGAGTGCTG. Internal right primer: CGAATTGGCTAAAAACTCGAA. Internal WT amplicon: 1192 bp. Deletion size: 336 bp. Deletion left flank: TATGTTCCATTGTTTGAAGAGTGCTGTTCT. Deletion right flank: TGAATAGAAAACGTCACGAAGTGGTGAGTT.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037642 Copy
http://www.wormbase.org/db/get?name=WBStrain00037641
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: available
References:
Synonyms: F48C1.4(ok3745) I.
Alternate IDs: WB-STRAIN:VC3041, CGC_VC3041
Notes: F48C1.4. External left primer: AACGATAGGAGACACGGTGG. External right primer: TGTGGTTGTTTTCGTTGCAT. Internal left primer: CAAGTTGAGAGTCCGCAGTG. Internal right primer: ACCATAAACTTGTTCGCGCT. Internal WT amplicon: 1143 bp. Deletion size: 523 bp. Deletion left flank: TTAGACAACTAACCATAGAGCGTGCAAATC. Deletion right flank: TGTTTCAGTGTTCTCCTTCCTGAAAAAAAA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037641 Copy
http://www.wormbase.org/db/get?name=WBStrain00037646
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000120(aly-1)
Genomic Alteration: WBGene00000120(aly-1)
Availability: available
References:
Synonyms: aly-1(ok3754) IV.
Alternate IDs: WB-STRAIN:VC3046, CGC_VC3046
Notes: C01F6.5. External left primer: CAACTCCCCCAAATTGGTAA. External right primer: GACGAAGGGATGATATGGGA. Internal left primer: TTTTTGATGTCACCTACCTATTCTA. Internal right primer: TTTGTTCGCCGTTCAATATG. Internal WT amplicon: 1251 bp. Deletion size: 617 bp. Deletion left flank: TCTCCAGATACTCCATCCACCTAGTCTATC. Deletion right flank: CGTGAACTTCAACGAGCACGGAAAACCAGT.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037646 Copy
http://www.wormbase.org/db/get?name=WBStrain00037647
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001072(dpy-10)|WBGene00001840(hel-1)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00001840(hel-1)
Availability: available
References:
Synonyms: hel-1(ok3698)/mT1 II; +/mT1 [dpy-10(e128)] III.
Alternate IDs: WB-STRAIN:VC3049, CGC_VC3049
Notes: C26D10.2. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok3698 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CAACCAAGTTCTGGCCATCT. External right primer: TTCCATTCTCCTTCCACCTG. Internal left primer: GGCGGAGAACATCATCACTT. Internal right primer: TTTCGGATCGTTTCGCTACT. Internal WT amplicon: 1141 bp. Deletion size: 721 bp. Deletion left flank: TGTCGCACTCGTCCAGGACGAAGTACTTGA. Deletion right flank: GAAATTTAGTAAATAACCTCACAAAAACAG.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037647 Copy
http://www.wormbase.org/db/get?name=WBStrain00037659
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006629(tsp-3)
Genomic Alteration: WBGene00006629(tsp-3)
Availability: available
References:
Synonyms: tsp-3(ok3729) III.
Alternate IDs: WB-STRAIN:VC3075, CGC_VC3075
Notes: Made_by: Vancouver KO Group|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y39E4B.4. External left primer: AAACCGCATTTGTCCGAATA. External right primer: TGCCCCCACTAACCAATATC. Internal left primer: TGTCTTAAAGCAAACGTGCAA. Internal right primer: ACTACTGCCGGCTCTATCGG. Internal WT amplicon: 1181 bp. Deletion size: 351 bp. Deletion left flank: GTTTTGATAAAGGCTTCGAATCGGAAATTC. Deletion right flank: AGGCTCCATACTTTCTGTTTATGGTTATCT."
Proper citation: RRID:WB-STRAIN:WBStrain00037659 Copy
http://www.wormbase.org/db/get?name=WBStrain00037654
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00009132(F25H8.2)
Genomic Alteration: WBGene00009132(F25H8.2)
Availability: available
References:
Synonyms: F25H8.2(ok3636) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC3062, CGC_VC3062
Notes: F25H8.2. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3636 homozygotes (sterile). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: ATGCAAACATGCTCCAATGA. External right primer: ATTATCCGATCTGGCAGGTG. Internal left primer: AACAAACGACACTCCGATTTC. Internal right primer: ATCTCGTTTTCGCCCTCTGT. Internal WT amplicon: 1333 bp. Deletion size: 333 bp. Deletion left flank: GAATGATTAGATTTCTAGCGTAATGTTCAC. Deletion right flank: AGAAGTTTTATAGGCATCGATGATACCCAT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037654 Copy
http://www.wormbase.org/db/get?name=WBStrain00037651
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00022645(irg-8)
Genomic Alteration: WBGene00022645(irg-8)
Availability: available
References:
Synonyms: irg-8(ok3738) V.
Alternate IDs: WB-STRAIN:VC3059, CGC_VC3059
Notes: Made_by: Vancouver KO Group|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZK6.11. External left primer: ATCAGTCATAAGGCGATGGG. External right primer: CCATTTCAATAAACCGGTCG. Internal left primer: AAGGCTTCAAGGCTGTCAGA. Internal right primer: GTGGCTCGGTTTCACACTTT. Internal WT amplicon: 1209 bp. Deletion size: 496 bp. Deletion left flank: CAGCGTACAGATATGCCAGAGCAGTAGAGT. Deletion right flank: TAAATATTATACGGCGGGTAAACTTTAAAA."
Proper citation: RRID:WB-STRAIN:WBStrain00037651 Copy
http://www.wormbase.org/db/get?name=WBStrain00037652
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003056(lon-2)|WBGene00007877(nfki-1)
Genomic Alteration: WBGene00003056(lon-2), WBGene00007877(nfki-1)
Availability: available
References:
Synonyms: +/szT1 [lon-2(e678)] I; C33A11.1(ok3681)/szT1 X.
Alternate IDs: WB-STRAIN:VC3060, CGC_VC3060
Notes: C33A11.1. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3681 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CCTGGTTGTCCTTTGCTGTT. External right primer: CTGTTACGCTGTGCTGGAAA. Internal left primer: ACATGGGTTTGTCCCTTTTT. Internal right primer: CCCCCATAATTTTCATATCACG. Internal WT amplicon: 1298 bp. Deletion size: 1022 bp. Deletion left flank: TCATTTTTATTTGAATCATCAACTTTTAAA. Deletion right flank: AGCTCAAGATGAAAAAAGAAAAAGAGCAGG. Insertion Sequence: ATATTTTGACTTCCTTTTTTATTTTTTTTTTCT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037652 Copy
http://www.wormbase.org/db/get?name=WBStrain00037657
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004740(scd-2)
Genomic Alteration: WBGene00004740(scd-2)
Availability: available
References:
Synonyms: scd-2(ok3702) V.
Alternate IDs: WB-STRAIN:VC3072, CGC_VC3072
Notes: Reference WBPaper00058832 added based on published strain data identified by Textpresso literature search.|"T10H9.2. External left primer: ATCACAAACCAATTGGGGAA. External right primer: TAATCCGGCTGGAAGAAATG. Internal left primer: CCCTGCGTATGCTAATTGGT. Internal right primer: TCCGGTCTAGTGGTAATCCG. Internal WT amplicon: 1147 bp. Deletion size: 660 bp. Deletion left flank: CTGATTTTATCGTTGAACGACGCGATAATC. Deletion right flank: CTTGTACAACATTACGTTTTTGATCTTCGC."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037657 Copy
http://www.wormbase.org/db/get?name=WBStrain00037658
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019362(cdk-2)
Genomic Alteration: WBGene00019362(cdk-2)
Availability: available
References:
Synonyms: cdk-2(ok3728) I.
Alternate IDs: WB-STRAIN:VC3074, CGC_VC3074
Notes: K03E5.3. External left primer: AAAATGCGTATTTCGCAACC. External right primer: AATTTCGTTCGATGACACCC. Internal left primer: CTTGTGTCGATTTACGGGCT. Internal right primer: TGAAGAGGAAAGACTCGGTAAAA. Internal WT amplicon: 1155 bp. Deletion size: 246 bp. Deletion left flank: GAATTAAAATAATTTATTAATTTAAATAAC. Deletion right flank: TTCCAAAAAAAAACATAAATTTCGATTATT. Insertion Sequence: CAAAAAAAAACATAAA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037658 Copy
http://www.wormbase.org/db/get?name=WBStrain00037661
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017353(F10E9.1)
Genomic Alteration: WBGene00017353(F10E9.1)
Availability: available
References:
Synonyms: F10E9.1(ok3764) III.
Alternate IDs: WB-STRAIN:VC3078, CGC_VC3078
Notes: F10E9.1. External left primer: AGCTGAAAAATGCTGTCGGT. External right primer: TTAAATGTGCAATGGTCCGA. Internal left primer: TACTGCACCACCGTTCAAAA. Internal right primer: CAGCTTCCTCATTTTCTGTTCTT. Internal WT amplicon: 1235 bp. Deletion size: 625 bp. Deletion left flank: AGTTGCTGGACAAAACAGCCGTGAGGAAGC. Deletion right flank: GGATACTTGAAATAAAAGGGAGCAGGAATC. Insertion Sequence: TTGAAATAAAA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037661 Copy
http://www.wormbase.org/db/get?name=WBStrain00037748
Source Database: WormBase (WB)
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: available
References:
Synonyms: F38F1A.1(gk3300) II.
Alternate IDs: WB-STRAIN:VC3254, CGC_VC3254
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y38F1A.1. External left primer: GCACCCCATTGTTGAACTTT. External right primer: ATGCCACGTAGCAAAAATCC. Internal left primer: TTCCCAAACACAAGAATCCC. Internal right primer: GCTAAGAGATATCGCGCGTC. Internal WT amplicon: 1616 bp. Deletion size: 493 bp. Deletion left flank: CTTCCCGGTGATTGTAAGGTCTTTAGACAT. Deletion right flank: TGTTTTTTATGTTGTTGTTTTTAAAATTGT. Validation: gk3300 passed by CGH."
Proper citation: RRID:WB-STRAIN:WBStrain00037748 Copy
http://www.wormbase.org/db/get?name=WBStrain00037741
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00009108(F25D1.3)|WBGene00012777(lact-8)|WBGene00014096(ZK829.7)|WBGene00015620(C08G9.2)
Genomic Alteration: WBGene00009108(F25D1.3), WBGene00012777(lact-8), WBGene00014096(ZK829.7), WBGene00015620(C08G9.2)
Availability: available
References:
Synonyms: C08G9.2(gk3191) ZK829.7(gk3253) IV; F25D1.3(gk3254) lact-8(gk3255) V.
Alternate IDs: WB-STRAIN:VC3245, CGC_VC3245
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain is homozygous for a deletion (gk3191) in C08G9.2, detectable by PCR using the following primers. External left primer: TCACAAGTTGGTACTGGGAGG. External right primer: CCATGCGAATTTTTGAACTGT. Internal left primer: ACAAGACCGTATGGGCAAAG. Internal right primer: ACCAATTTCATCTTGCCCTG. Internal WT amplicon: 1980 bp. Deletion size: 465 bp. Deletion left flank: CATTTCAAAAATCCATGGCAATCCGAATCT. Deletion right flank: ATCACCGTATCCACTGTTTTTGCAATGGTA. Validation: gk3191 passed by CGH. Other deletions (gk3253, gk3254, gk3255) identified by CGH."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037741 Copy
http://www.wormbase.org/db/get?name=WBStrain00037746
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00008474(nhr-174)
Genomic Alteration: WBGene00008474(nhr-174)
Availability: available
References:
Synonyms: nhr-174(gk3192) I.
Alternate IDs: WB-STRAIN:VC3252, CGC_VC3252
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain is homozygous for a deletion (gk3192) in E03H4.6, detectable by PCR using the following primers. External left primer: ACAGGGCGATTGACGATAAC. External right primer: CAGATAACCATGTCCCCCAC. Internal left primer: CCTCCAAACAATCCTCAAACA. Internal right primer: CTACGGAATGAATTGGCTTCA. Internal WT amplicon: 1592 bp. Deletion size: 392 bp. Deletion left flank: AGAGGTATGTTAAAACGTATGTATGTATGT. Deletion right flank: TTGGATTGAATCTGCATGGAATTATTTGAT. Validation: gk3192 passed by CGH with slightly low log2 scores."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037746 Copy
http://www.wormbase.org/db/get?name=WBStrain00037743
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019561(K09C6.7)|WBGene00043067(dlc-5)
Genomic Alteration: WBGene00019561(K09C6.7), WBGene00043067(dlc-5)
Availability: available
References:
Synonyms: dlc-5(gk3510) IV; K09C6.7(gk3297) V.
Alternate IDs: WB-STRAIN:VC3247, CGC_VC3247
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain is homozygous for a deletion (gk3297) in K09C6.7, detectable by PCR using the following primers. External left primer: GGCGGTGGTCCAGTAAACTA. External right primer: GCTCGGTTTTACGGAATTGA. Internal left primer: GTTGACGCCTCGACATGTAA. Internal right primer: CAGGAACGTTGCCAGGTAAT. Internal WT amplicon: 2481 bp. Deletion size: 2123 bp. Deletion left flank: GAAATGTTGACGCCTCGACATGTAAGTGTT. Deletion right flank: TTTTCAAAATTTCTACATTTCTGTACTAAT. Insertion Sequence: T. Validation: gk3297 passed by CGH. Other deletion (gk3510) identified by CGH."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037743 Copy
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