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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00037521
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020048(lgc-12)
Genomic Alteration: WBGene00020048(lgc-12)
Availability: available
References:
Synonyms: lgc-12(ok3546) III.
Alternate IDs: WB-STRAIN:VC2740, CGC_VC2740
Notes: R13A5.4. External left primer: AGCGAGAGCTGGTGAAACAT. External right primer: CTCGGACAATTTTCGCGTAT. Internal left primer: CCAATTTACTCGACCTGTAAAAA. Internal right primer: TGCATCAAATTAGGTGTCCG. Internal WT amplicon: 1216 bp. Deletion size: 744 bp. Deletion left flank: ACGTAAGTTATGGTAAATAACATACTTTTT. Deletion right flank: GCAATACCGTTCCAGCATTTTCACAGTTAA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037521 Copy
http://www.wormbase.org/db/get?name=WBStrain00037522
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001488(frm-1)
Genomic Alteration: WBGene00001488(frm-1)
Availability: available
References:
Synonyms: frm-1(gk1225) I.
Alternate IDs: WB-STRAIN:VC2741, CGC_VC2741
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZK270.2. Identified by PCR, validated by CGH. External left primer: AATGGTGACACGATGCTCAA. External right primer: ACACAGACACAGCAAGACGG. Internal left primer: GTTAAATTCCAGTGGCTGCG. Internal right primer: GAAGCCGATGGACAAAGAGA. Internal WT amplicon: 796 bp. Deletion size: 98 bp. Deletion left flank: AAGTGATCATTCGACCTTTAAAAGTGATGT. Deletion right flank: TTTGGGTGTACCAGTTAGATATATTGGGGT."
Proper citation: RRID:WB-STRAIN:WBStrain00037522 Copy
http://www.wormbase.org/db/get?name=WBStrain00037520
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003056(lon-2)|WBGene00006839(unc-115)
Genomic Alteration: WBGene00003056(lon-2), WBGene00006839(unc-115)
Availability: available
References:
Synonyms: +/szT1 [lon-2(e678)] I; unc-115(ok2640)/szT1 X.
Alternate IDs: WB-STRAIN:VC2739, CGC_VC2739
Notes: F09B9.2. Homozygous viable deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok2640 homozygotes (Unc). Pick WT and check for correct segregation of progeny to maintain. External left primer: TCTCATTTTGGTGACGGTGA. External right primer: AAAGGGCAATGAGTTTGCAC. Internal left primer: AGACGAGATCTGGCATCCAT. Internal right primer: GAGAAGAAGAAAAGGCGCAC. Internal WT amplicon: 1358 bp. Deletion size: 512 bp. Deletion left flank: GCAGAATAAAAATTAAAAAAAAATGTTTAA. Deletion right flank: TTGAATCAGTAGCTGGCTATAGAGCACAAC.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037520 Copy
http://www.wormbase.org/db/get?name=WBStrain00037525
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00018488(acs-1)
Genomic Alteration: WBGene00018488(acs-1)
Availability: available
References:
Synonyms: acs-1(gk3066) V/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2744, CGC_VC2744
Notes: F46E10.1. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP gk3066 homozygotes (early larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CTTCGATCAGCAGTTGACCA. External right primer: CAAAGTTGGCAATGGTTGTG. Internal left primer: CAACACAGTTTGCCAGTGCT. Internal right primer: GAGACGACTTGCTGGAGACC. Internal WT amplicon: 2067 bp. Deletion size: 880 bp. Deletion left flank: TTTATTTTAAAAAATATTTAAAAAGTTTTA. Deletion right flank: TATGACTGACATGCAAGTATGCTATGGAAC.|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037525 Copy
http://www.wormbase.org/db/get?name=WBStrain00037526
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001063(dpy-1)|WBGene00002025(hsp-60)
Genomic Alteration: WBGene00001063(dpy-1), WBGene00002025(hsp-60)
Availability: available
References:
Synonyms: hsp-60(ok3508)/sC1 [dpy-1(s2170)] III.
Alternate IDs: WB-STRAIN:VC2754, CGC_VC2754
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y22D7AL.5. Apparent homozygous lethal deletion chromosome balanced by dpy-1-marked recombination suppressor. Heterozygotes are WT, and segregate WT, Dpy (sC1 homozygotes), and ok3508 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: AAATTGATTTTTCCCGCTGA. External right primer: AGGGGAAAAAGAGCCGTAAA. Internal left primer: GAAATTTTGGTTTTCCTGCG. Internal right primer: CAAATGGCTCAGAGCACAAA. Internal WT amplicon: 1227 bp. Deletion size: 611 bp. Deletion left flank: AAAAATTTGAATTTTTCGTGAAAATTTGAA. Deletion right flank: GCTCTCAATCTCTCATTGAAATAACGACAC."
Proper citation: RRID:WB-STRAIN:WBStrain00037526 Copy
http://www.wormbase.org/db/get?name=WBStrain00037538
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001072(dpy-10)|WBGene00003928(pas-7)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00003928(pas-7)
Availability: available
References:
Synonyms: pas-7(ok3447)/mIn1 [mIs14 dpy-10(e128)] II.
Alternate IDs: WB-STRAIN:VC2777, CGC_VC2777
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"ZK945.2. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok3447 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: TGTGATGATCGAGGAAGCAG. External right primer: TTCGTCTCTCCCGTAAATCG. Internal left primer: AAGCAGTTGCCGCATAACTT. Internal right primer: AACGGTTCTTCTGATTTCCG. Internal WT amplicon: 1263 bp. Deletion size: 409 bp. Deletion left flank: GAATTGTGCATAAACATGTTTCTGGTTTGT. Deletion right flank: ATTCACATCCAGCTCCTCGATCTTCAGCTT."
Proper citation: RRID:WB-STRAIN:WBStrain00037538 Copy
http://www.wormbase.org/db/get?name=WBStrain00037533
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00012731(Y39G8C.2)
Genomic Alteration: WBGene00012731(Y39G8C.2)
Availability: available
References:
Synonyms: Y39G8C.2(gk1099) II.
Alternate IDs: WB-STRAIN:VC2771, CGC_VC2771
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y39G8C.2, K03H6.5. External left primer: AAAGAGTTGAAGGCAGGCAA. External right primer: TGAGGAAAATGACCGAAAGG. Internal left primer: GTCGAGCAGCAGGTAGAACC. Internal right primer: GCAAGTTTCCGGAATTGAAA. Internal WT amplicon: 2604 bp. Deletion size: 1081 bp. Deletion left flank: CGCCGGCGCTCGAGCGTTTTAGCGTGCCGA. Deletion right flank: CTTTGTGTACTGCGGCCGACGCTGCACGGG."
Proper citation: RRID:WB-STRAIN:WBStrain00037533 Copy
http://www.wormbase.org/db/get?name=WBStrain00037537
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001072(dpy-10)|WBGene00004392(rnr-2)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00004392(rnr-2)
Availability: available
References:
Synonyms: +/mT1 II; rnr-2(ok3357)/mT1 [dpy-10(e128)] III.
Alternate IDs: WB-STRAIN:VC2776, CGC_VC2776
Notes: C03C10.3. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok3357 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: GTCTCTCGGCTTCATTCACC. External right primer: GTGTAAAGTCCGCGAAGAGG. Internal left primer: ATACTCGGAAACCCGCTTCT. Internal right primer: ATGCCTTCGAATTTACAGCC. Internal WT amplicon: 1159 bp. Deletion size: 691 bp. Deletion left flank: TTCGATGGCCACAGCGTCCTTGATGATATC. Deletion right flank: TGCCTTTTTGTAGAAGTTCCAGATGTCATG. Insertion Sequence: ATTGATGA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037537 Copy
http://www.wormbase.org/db/get?name=WBStrain00037620
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006698(uaf-2)
Genomic Alteration: WBGene00006698(uaf-2)
Availability: available
References:
Synonyms: uaf-2(gk3159) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC3010, CGC_VC3010
Notes: Homozygous lethal deletion chromosome (gk3159 in Y116A8C.35) balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP gk3159 homozygotes (early larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TTCTGAGCAGTTTGCAGGTG. External right primer: TTTCTGTAAAAATTGGCCGC. Internal left primer: CTCCATATCCGTAGCCTCCA. Internal right primer: GATGCAAGAGACGCAGAGAA. Internal WT amplicon: 2193 bp. Deletion size: 871 bp. Deletion left flank: CCGCCTCCGGAACCTCCACGTTGTGATGGA. Deletion right flank: AGTGGCACGTTCTCTTCACAGCACTTGAGC. Insertion Sequence: G.|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037620 Copy
http://www.wormbase.org/db/get?name=WBStrain00037621
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00013149(Y53F4B.1)
Genomic Alteration: WBGene00013149(Y53F4B.1)
Availability: available
References:
Synonyms: gkDf21 I; Y53F4B.1(gk1289) II.
Alternate IDs: WB-STRAIN:VC3011, CGC_VC3011
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain is homozygous for a deletion (gk1289) in Y53F4B.1, detectable by PCR using the following primers. External left primer: GCACTTCAAACGCAGATTCA. External right primer: GTTGTGGCTGCTCTGAACAA. Internal left primer: GCTGCTGACGTCACACTGAT. Internal right primer: TATTGGTGAAAGAGAGGCCG. Internal WT amplicon: 2011 bp. Deletion size: 862 bp. Deletion left flank: AATAGAAGGTAGGCAGGCACGTAGGCAGCG. Deletion right flank: AATTTGCCGTTTGCCAGAAATGTTTTTTTT. Validation: gk1289 passed by CGH. Other deletion (gkDf21) identified by CGH."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037621 Copy
http://www.wormbase.org/db/get?name=WBStrain00037624
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00008502(skih-2)|WBGene00022705(ZK354.2)
Genomic Alteration: WBGene00008502(skih-2), WBGene00022705(ZK354.2)
Availability: available
References:
Synonyms: ZK354.2(gk1288) F01G4.3(gk3110) IV.
Alternate IDs: WB-STRAIN:VC3016, CGC_VC3016
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain is homozygous for a deletion (gk1288) in ZK354.2, detectable by PCR using the following primers. External left primer: GCCTCCCCCTATCGATAAAC. External right primer: TCGTCTTGTTGTTCTTCCCC. Internal left primer: TGAACATGAAGAGCTCGGTG. Internal right primer: GTACCCGGGACCCTTGTAAT. Internal WT amplicon: 1294 bp. Deletion size: 770 bp. Deletion left flank: AACATGAAGAGCTCGGTGAGTTATTGATGG. Deletion right flank: CCAAGAAAAACGATGAAGCTGAGGAGCAGA. Validation: gk1288 passed by CGH. Other deletion (gk3110) identified by CGH."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037624 Copy
http://www.wormbase.org/db/get?name=WBStrain00037632
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020195(T03G6.3)
Genomic Alteration: WBGene00020195(T03G6.3)
Availability: available
References:
Synonyms: T03G6.3(ok3710) X.
Alternate IDs: WB-STRAIN:VC3028, CGC_VC3028
Notes: Made_by: Vancouver KO Group|"T03G6.3. External left primer: GGTGAATTCTCAGTGCACCA. External right primer: CGGAAAAGCTGGAGTAGACG. Internal left primer: ACTTAGAGTTGCCGACCAGG. Internal right primer: TTATTGGTTTGCACATTGCC. Internal WT amplicon: 1269 bp. Deletion size: 572 bp. Deletion left flank: TTGTTCCTGGCTTTGTAATCAGTACAACAC. Deletion right flank: AAGCATCGGTGGTTCAGTGGTAGAATGCTC."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037632 Copy
http://www.wormbase.org/db/get?name=WBStrain00037630
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00016890(lst-5)
Genomic Alteration: WBGene00016890(lst-5)
Availability: available
References:
Synonyms: C52E12.6(ok3724) II.
Alternate IDs: WB-STRAIN:VC3026, CGC_VC3026
Notes: C52E12.6. External left primer: GAAAAGAGAAGCAGCCATGC. External right primer: CGTTTTGCTGAAGAAGGAGG. Internal left primer: ATTTCCAGATTGCTCACGCT. Internal right primer: TACCCTCCATAAACCACCGA. Internal WT amplicon: 1156 bp. Deletion size: 585 bp. Deletion left flank: GATGCACATGGATATTTGGGTATGTGTGAC. Deletion right flank: AAAGTTTAGGTTTAATAGGGTAATACACAA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037630 Copy
http://www.wormbase.org/db/get?name=WBStrain00037635
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003530(nas-11)
Genomic Alteration: WBGene00003530(nas-11)
Availability: available
References:
Synonyms: nas-11(ok3723) X.
Alternate IDs: WB-STRAIN:VC3032, CGC_VC3032
Notes: K11G12.1. External left primer: AAAACACAGGCACCTTGGTC. External right primer: TCTGATTGGGGAACTTGGAT. Internal left primer: CAAAGAATGGAAAGGCAAAG. Internal right primer: ACTAGGATGAGATGGGCAGC. Internal WT amplicon: 1336 bp. Deletion size: 982 bp. Deletion left flank: TCATGTAAGCTCGGAACATGTGAACAAACT. Deletion right flank: AAAACGGGCAGAATTGTAGATTTGCTGCCC.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037635 Copy
http://www.wormbase.org/db/get?name=WBStrain00037633
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001072(dpy-10)|WBGene00004304(ran-3)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00004304(ran-3)
Availability: available
References:
Synonyms: ran-3(ok3709)/mIn1 [mIs14 dpy-10(e128)] II.
Alternate IDs: WB-STRAIN:VC3029, CGC_VC3029
Notes: C26D10.1. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok3709 homozygotes (early- to mid-larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: TCGTCTTTCAATCCGAGACC. External right primer: ATTGGCGATCGAGTTTTGTC. Internal left primer: GGCAGAAACACCAACGATCT. Internal right primer: AAAAAGCCACGGAAAGTTGA. Internal WT amplicon: 1104 bp. Deletion size: 592 bp. Deletion left flank: TCCGAAGGCGTAGTATTTTCCGTCTTCTCC. Deletion right flank: CTTCCTTCCTTCTCTACACCTTCCGCGGGA. Insertion Sequence: CTTTTTTTCCTTTTTTTTCCGTCTT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037633 Copy
http://www.wormbase.org/db/get?name=WBStrain00037606
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001078(dpy-19)|WBGene00001609(glp-1)|WBGene00020102(R148.3)
Genomic Alteration: WBGene00001078(dpy-19), WBGene00001609(glp-1), WBGene00020102(R148.3)
Availability: available
References:
Synonyms: R148.3(ok3525)/qC1 [dpy-19(e1259) glp-1(q339)] III.
Alternate IDs: WB-STRAIN:VC2972, CGC_VC2972
Notes: R148.3. Apparent homozygous lethal deletion chromosome balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and ok3525 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TGAGACAACAGTGAGCCGAC. External right primer: GCTGCCTTCCATGACTTCTC. Internal left primer: CTCATGCTCAACGTCAGGAA. Internal right primer: TGTCGATCGTCTTCTCATCG. Internal WT amplicon: 1190 bp. Deletion size: 862 bp. Deletion left flank: GACGGCGGAGAATCGAGATTTGACAGATAA. Deletion right flank: TCATCGATGAGAAGACGATCGACACGTCGG.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037606 Copy
http://www.wormbase.org/db/get?name=WBStrain00037604
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004399(rol-9)|WBGene00006652(ttx-1)|WBGene00006786(unc-51)
Genomic Alteration: WBGene00004399(rol-9), WBGene00006652(ttx-1), WBGene00006786(unc-51)
Availability: available
References:
Synonyms: ttx-1(ok2889)/unc-51(e369) rol-9(sc148) V.
Alternate IDs: WB-STRAIN:VC2961, CGC_VC2961
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y113G7A.6. Apparent homozygous lethal deletion chromosome balanced by flanking markers. Heterozygotes are WT and segregate WT, Unc-51 Rol-9 homozygotes and ok2889 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TCTCGGGGAGTTGAATTTTG. External right primer: TTTTTCCCGAATTTTTGCAC. Internal left primer: ATGTCTTCCCGCATGAAAAT. Internal right primer: CCAGTGGTCAGAAAGCCAAT. Internal WT amplicon: 1294 bp. Deletion size: 888 bp. Deletion left flank: GTTGTTTTCTAGAAAATCTGAAAATTTTTA. Deletion right flank: TTACGAATATGAAATTTATCAAGGTCTAGG."
Proper citation: RRID:WB-STRAIN:WBStrain00037604 Copy
http://www.wormbase.org/db/get?name=WBStrain00037608
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004115(pqn-26)
Genomic Alteration: WBGene00004115(pqn-26)
Availability: available
References:
Synonyms: pqn-26(ok3706) I.
Alternate IDs: WB-STRAIN:VC2974, CGC_VC2974
Notes: DY3.5. External left primer: ACCCGAGTAGTTGGTGATGG. External right primer: GCAACTTATCCGCCAACATT. Internal left primer: TGGTACAACCGATGAGCTTG. Internal right primer: GCGCTTGGCATTTCTAAAGT. Internal WT amplicon: 1109 bp. Deletion size: 528 bp. Deletion left flank: ACCACTTGTTGTTGAGATATAACTGATCCA. Deletion right flank: GCGAGTTGTTGCTGTTGGGCAATCTAAAGT. Insertion Sequence: GCCTGTTGAGCTGCGATTTGTTCC.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037608 Copy
http://www.wormbase.org/db/get?name=WBStrain00037609
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006815(unc-83)|WBGene00019141(bath-5)|WBGene00021525(cpg-24)
Genomic Alteration: WBGene00006815(unc-83), WBGene00019141(bath-5), WBGene00021525(cpg-24)
Availability: available
References:
Synonyms: bath-5(gk3138) II; Y41D4B.26(gk1259) IV; unc-83(gk3139) V.
Alternate IDs: WB-STRAIN:VC2975, CGC_VC2975
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"W01A11.3, Y41D4B.26, F07E5.7. The gk1259 allele was identified by PCR and validated by CGH, and can be detected with PCR using the following primers. External left primer: AGAGTTCGGGGCTGATTTTT. External right primer: AGGAGGGACTTTTTAGGCCA. Internal left primer: AACTGAGCCACTCGGGTAAA. Internal right primer: TGCTGATTGGAAGAAGTGGA. Internal WT amplicon: 2165 bp. Deletion size: 1624 bp. Deletion left flank: CTGAGCCACTCGGGTAAAACTAAATTTTTT. Deletion right flank: ATTTTTTTCTAGAAACTGGACCGGCGAAAA. Insertion Sequence: CCCTTTCCCCCC. Other lesions identified by CGH."
Proper citation: RRID:WB-STRAIN:WBStrain00037609 Copy
http://www.wormbase.org/db/get?name=WBStrain00037684
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00002204(kin-21)
Genomic Alteration: WBGene00002204(kin-21)
Availability: available
References:
Synonyms: kin-21(gk3184) IV.
Alternate IDs: WB-STRAIN:VC3123, CGC_VC3123
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain is homozygous for a deletion (gk3184) in W08D2.8, detectable by PCR using the following primers. External left primer: TGAACCATTTCACTAGCCCC. External right primer: GCTCTATCCGTTCTTCGTGC. Internal left primer: AATGATGTTCGGAAAGGCTG. Internal right primer: CATTCGGGAGTAGATGCGAT. Internal WT amplicon: 2184 bp. Deletion size: 652 bp. Deletion left flank: ATTCTCCAAAGGATTATTCAATGAGAAAAC. Deletion right flank: CTAAGTGAACTCATGTAATCAACAAAATAG. Validation: gk3184 passed by CGH."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037684 Copy
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